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Merck

P7914

Phthaldialdehyde Reagent

Solution Incomplete

Synonym(s):

OPA

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About This Item

CAS Number:
643-79-8
NACRES:
NA.21
PubChem Substance ID:
24898893
UNSPSC Code:
12352201
MDL number:
MFCD00003335

Key Documents

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Product Name

Phthaldialdehyde Reagent, Solution Incomplete

InChI

1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H

SMILES string

[H]C(=O)c1ccccc1C([H])=O

InChI key

ZWLUXSQADUDCSB-UHFFFAOYSA-N

storage temp.

2-8°C

Quality Level

200

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Application

Phthaldialdehyde Reagent has been used for the derivatization plasma samples prior to high performance liquid chromatography.
Precolumn derivatization reagent for primary amines and amino acids. The fluorescent derivative can be detected by reverse-phase HPLC. The reaction requires OPA, primary amine and a sulfhydryl. In the presence of excess sulfhydryl, amines can be quantitated. In the presence of excess amine, sulfhydryls can be quantitated.
Useful for investigators requiring an alternate sulfhydryl moiety. Requires addition of desired sulfhydryl compound for measuring amines. Requires addition of amine to measure sulfhydryls.

Other Notes

Same as P 0532, but contains no 2-mercaptoethanol.

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1B

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000448514
0000448514
0000427893
0000427893
0000354521

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Low-dose tryptophan depletion in recovered depressed patients induces changes in cognitive processing without depressive symptoms
Hayward G, et al.
Biological Psychiatry, 57(5), 517-524 (2005)
Yi-Jhen Lai et al.
Talanta, 91, 103-109 (2012-03-01)
This study reports a selective and sensitive method for fluorescent detection of total, protein-bound, free, and free oxidized homocysteine (HCys) using tris(2-carboxyethyl)phosphine (TCEP) as a reducing agent, fluorosurfactant-capped gold nanoparticles (FSN-AuNP) as a preconcentrating probe, and o-phthaldialdehyde (OPA) as a
Atsuo Iwasawa et al.
Biocontrol science, 16(4), 165-170 (2011-12-23)
We investigated the bactericidal effects and cytotoxicity of an ortho-phthalaldehyde product in comparison with those of its predecessor glutaraldehyde products. Bactericidal effects ware examined on Mycobacterium terrae, a standard organism used for investigating the bactericidal effect of high-level disinfectants. Cytotoxicity
Katsuhiro Suzuki et al.
Biomedical chromatography : BMC, 27(4), 535-538 (2012-09-29)
Monomethylarginine, asymmetric dimethylarginine and symmetric dimethylarginine were separated on a Wakopak Combi ODS with an acetonitrile-100 mm potassium phosphate buffer (pH 7.0; 1:1, v/v). Dimethylarginines were derived from o-phthalaldehyde for the fluorescence detector and from 6-ferrocenyl-1-hexanethiol for the electrochemical detector. The
Noriko Nishino et al.
Environmental science & technology, 46(15), 8198-8204 (2012-07-20)
Naphthalene, typically the most abundant polycyclic aromatic hydrocarbon in the atmosphere, reacts with OH radicals by addition to form OH-naphthalene adducts. These OH-naphthalene adducts react with O(2) and NO(2), with the two reactions being of equal importance in air at
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