T17917

Tricine

Name: Tricine
Brand: SIAL

≥99% (titration), reagent grade, crystalline

Synonym(s):

N-[Tris(hydroxymethyl)methyl]glycine

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About This Item

Linear Formula:

(HOCH₂)₃CNHCH₂CO₂H

CAS Number:

5704-04-1

Molecular Weight:

179.17

NACRES:

NA.25

UNSPSC Code:

12161700

Beilstein/REAXYS Number:

1937804

MDL number:

MFCD00004277

Product Name

Tricine, ≥99% (titration), reagent grade, crystalline

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

biological source

synthetic

grade

reagent grade

assay

≥99% (titration)

form

crystalline

storage condition

(Tightly closed. Dry)

impurities

<0.5% water

color

white

useful pH range

7.4-8.8

pKa (25 °C)

8.1

mp

187 °C

solubility

water: 0.1 M, clear, colorless

cation traces

Pb: ≤5 ppm

storage temp.

room temp

Quality Level

200

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Related Categories

Biochemicals

Biological Buffers

Chemistry & Biochemicals

Application

Buffer component for separation of low molecular weight peptides.

Tricine is intended to use for:

Electrophoresis To separate low-weight peptides and proteins
Crystallization of proteins of varying pH
Resuspension of cell pellets
Enzyme assays
Tissue culture

Features and Benefits

Ideal as a Biological buffer for Cell Biology and Biochemical research
Effective Buffering from pH 7.4 - 8.8 with a pKa of 8.1 (25 °C)

General description

Tricine, a zwitterionic biological buffer, is commonly employed in research applications such as enzyme assays, electrophoresis, cell biology, and biochemical studies. Its increased negative charge compared to glycine ensures rapid migration during electrophoresis, facilitating efficient separation of low-molecular-weight proteins in gels with lower acrylamide percentages. Moreover, Tricine may also be used in cryopreservation media, aiding in the preservation of tissues and organs.

Tricine′s valuable properties for biological research include its zwitterionic nature, wide buffering range (pH 7.4-8.8), classification as a Good′s buffering agent, non-toxicity to cells, and high water solubility and clarity. These attributes make it suitable for metal-sensitive systems, stable pH maintenance, optimal biological applications, cell biology studies, and accurate measurements. Additionally, Tricine may find utilization in capillary zone electrophoresis, high-performance liquid chromatography, and ion exchange chromatography, demonstrating adaptability across analytical techniques.

Other Notes

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

H Schägger et al.

Analytical biochemistry, 166(2), 368-379 (1987-11-01)

A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations

Tricine-SDS-PAGE.

Hermann Schägger

Nature protocols, 1(1), 16-22 (2007-04-05)

Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in