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Merck

14385C

EX-CELL® CD HEK293 Viral Vector Medium

Chemically defined, animal component-free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

HEK293/HEK293T medium for viral vector production

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About This Item

UNSPSC Code:
12352207
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Quality Segment

sterility

sterile-filtered

product line

EX-CELL®

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

L-glutamine: no

shipped in

wet ice

storage temp.

2-8°C

General description

EX-CELL CD HEK293 Viral Vector Medium is an animal origin-free, chemically defined cell culture medium for clinical and commercial manufacturing of viral vectors for gene and cell therapies. The medium is intended for processes implementing (polyethylenimine) PEI transfection reagents.

Application

  • EX-CELL CD HEK293 Viral Vector Medium is part of the VirusExpress® LentiviralProduction Platform and the medium of choice for VirusExpress 293T LentiviralProduction Cells (VP001).
  • The medium is also suitable as a standalone solution for other HEK293 and HEK293T cell lines for AAV and lentiviral manufacturing.
  • EX-CELL CD HEK293 Viral Vector Medium has been formulated tosupport high suspension cell densities with minimal clumping, high transienttransfection efficiency when used with PEI, and high viral titers in shakeflask and stirred tank bioreactor formats
  • The following white paper demonstrates the use of oneof the prototype media formulations in developing a scalable, transienttransfection-based suspension process for lentiviral vector production usingthe VirusExpress 293T Lentiviral Production cells:

Features and Benefits

Viral vector productionusing this medium is a scalable, suspension process that does not require amedia exchange or feed post-transfection.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1



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