A3553

Acrylamide

Name: Acrylamide
Brand: SIGMA

suitable for electrophoresis, ≥99% (HPLC), powder

Synonym(s):

2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide

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All Photos(4)

About This Item

Linear Formula:

CH₂=CHCONH₂

CAS Number:

79-06-1

Molecular Weight:

71.08

NACRES:

NB.22

PubChem Substance ID:

24890783

eCl@ss:

39031205

UNSPSC Code:

41105319

EC Number:

201-173-7

MDL number:

MFCD00008032

Beilstein/REAXYS Number:

605349

Product Name

Acrylamide, suitable for electrophoresis, ≥99% (HPLC), powder

cation traces

Fe: ≤1 ppm
Mg: ≤3 ppm
heavy metals (as Pb): ≤5 ppm

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

SMILES string

NC(=O)C=C

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99% (HPLC)

form

powder

technique(s)

electrophoresis: suitable

impurities

≤0.005% Acrylic acid
≤0.005% Insoluble in water

conductivity

≤10 μmho (35% solution)

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

Quality Level

200

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Application

Acrylamide has been used in the preparation of polyacrylamide gels.

General description

Acrylamide functions as a quencher of tryptophan fluorescence. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers. Acrylamide alkylates SH groups of cysteine and ε-NH₂ lysine residues of proteins during electrophoresis.

Other Notes

This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product.

pictograms

GHS06,GHS08

signalword

Danger

hcodes

H301,H312 + H332,H315,H317,H319,H340,H350,H361f,H372

pcodes

P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

280.4 °F - closed cup

flash_point_c

138 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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The Protein Protocols Handbook (2007)

Chemistry, biochemistry, and safety of acrylamide. A review

Friedman M

Journal of Agricultural and Food Chemistry, 51(16), 4504-4526 (2003)

Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions

Chiang MYM, et al.

Biomaterials, 34(38), 9754-9762 (2013)

Composite three-dimensional woven scaffolds with interpenetrating network hydrogels to create functional synthetic articular cartilage.

I-Chien Liao et al.

Advanced functional materials, 23(47), 5833-5839 (2014-03-01)

The development of synthetic biomaterials that possess mechanical properties that mimic those of native tissues remains an important challenge to the field of materials. In particular, articular cartilage is a complex nonlinear, viscoelastic, and anisotropic material that exhibits a very

Acrylamide concentration determines the direction and magnitude of helical membrane protein gel shifts.

Arianna Rath et al.

Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)

SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise

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