A5670
Anti-Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Pig Antibodies, Anti-Pig Antibodies - Anti-Pig IgG (whole molecule)-Peroxidase antibody produced in rabbit, Anti-Porcine IgG (whole molecule)−Peroxidase antibody produced in rabbit, Anti-Swine IgG (whole molecule)−Peroxidase antibody produced in rabbit
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About This Item
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d)
Citations:
22
biological source
rabbit
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
pig
technique(s)
direct ELISA: 1:40,000, dot blot: 1:150,000 (chemiluminescent)
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
Immunoglobulin is a glycoprotein, with two heavy chain and two light chain connected by disulfide bond. IgG is a major class of immunoglobulin. It has five subclasses of IgG- IgG1, IgG3, IgG2a and IgG2b. Affinity isolated antibody is obtained from rabbit anti-pig IgG antiserum by immunospecific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to pig IgG. Anti-Pig IgG is then conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde.
Immunogen
purified pig IgG
Application
Anti-Pig IgG (whole molecule)?Peroxidase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It mainly helps in immune defense. Pig IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-pig IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Rabbit anti-pig IgG (whole molecule)-peroxidase antibody binds to pig IgG.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Jesús Horacio Lara-Puente et al.
mBio, 12(5), e0190821-e0190821 (2021-09-22)
Vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were developed in record time and show excellent efficacy and effectiveness against coronavirus disease 2019 (COVID-19). However, currently approved vaccines cannot meet the global demand. In addition, none of the currently
P S Pinto et al.
Revista do Instituto de Medicina Tropical de Sao Paulo, 42(2), 71-79 (2000-05-16)
In the present study ELISA was standardized for the diagnosis of swine cysticercosis based on necropsy parameters and confirmed positive and negative control sera. Serum samples from pigs with other infections were also assayed to determine possible cross-reactions. Four antigens
Abbey Olsen et al.
Veterinary parasitology, 295, 109460-109460 (2021-05-25)
Serological tests are routinely used to detect Toxoplasma gondii specific IgG antibodies in serum. Serological surveys of T. gondii show a medium to high prevalence in Danish indoor sows at the time of slaughter. However, little is known about when
Júlia Vergara-Alert et al.
Emerging infectious diseases, 23(2), 232-240 (2016-12-03)
Middle East respiratory syndrome (MERS) cases continue to be reported, predominantly in Saudi Arabia and occasionally other countries. Although dromedaries are the main reservoir, other animal species might be susceptible to MERS coronavirus (MERS-CoV) infection and potentially serve as reservoirs.
Livestock susceptibility to infection with Middle East respiratory syndrome coronavirus
Vergara AJ, et al.
Emerging Infectious Diseases, 23(2), 232-232 (2017)
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