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Merck

A8181

Amido Black Staining Solution 2X

electrophoresis reagent

Synonym(s):

Naphthol Blue Black solution, Amido Black 10B

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About This Item

Empirical Formula (Hill Notation):
C22H14N6Na2O9S2
CAS Number:
1064-48-8
Molecular Weight:
616.49
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
24891210
MDL number:
MFCD00004017
Beilstein/REAXYS Number:
5374263

Key Documents

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Product Name

Amido Black Staining Solution 2X, electrophoresis reagent

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;

SMILES string

[Na+].[Na+].Nc1c(\N=N/c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\C=C/c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O

InChI key

AOMZHDJXSYHPKS-DROYEMJCSA-L

form

liquid

Quality Level

200

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Application

Amido Black Staining Solution 2X has been used in the staining:
  • serum proteins present in the culture supernatant
  • cysteine sulfinic acid decarboxylase protein in mouse liver homogenate
  • histones from chromatin samples

General description

Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints.

Preparation Note

After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

53.6 °F

flash_point_c

12 °C

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Certificates of Analysis (COA)

Lot/Batch Number
0000420539
0000420539
SLCP7209
SLCK9090
SLCF5171

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Detection of proteins on blot membranes
Goldman A, et al.
Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [Et Al.], 86(1), 10-18 (2016)
Placental release of taurine to both the maternal and fetal circulations in human term pregnancies
Holm MB, et al.
Amino Acids, 50(9), 1205-1214 (2018)
Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter.
J M Gershoni et al.
Analytical biochemistry, 124(2), 396-405 (1982-08-01)
Meghan C Hughes et al.
The Journal of physiology, 598(7), 1377-1392 (2019-01-24)
Ninety-eight per cent of patients with Duchenne muscular dystrophy (DMD) develop cardiomyopathy, with 40% developing heart failure. While increased propensity for mitochondrial induction of cell death has been observed in left ventricle, it remains unknown whether this is linked to
Protein Blotting and detection: methods and protocols, 86(1), 10-18 (2009)
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