A8706

Avidin from egg white

Name: Avidin from egg white
Brand: SIGMA

recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)

Synonym(s):

AVID_CHICK

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About This Item

CAS Number:

1405-69-2

UNSPSC Code:

12352202

EC Number:

215-783-6

NACRES:

NA.56

MDL number:

MFCD00130572

Product Name

Avidin from egg white, recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)

recombinant

expressed in corn

assay

≥80% protein basis (biuret)

form

powder

specific activity

≥12 units/mg protein (E1%/280)

mol wt

glycoprotein 66 kDa
subunit 16 kDa

technique(s)

ELISA: suitable
immunoblotting: suitable
immunoelectrophoresis: suitable

UniProt accession no.

P02701

storage temp.

2-8°C

Quality Level

200

Gene Information

chicken ... AVD(396260)

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Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.

Avidin forms an extremely strong complex with d-biotin (K_d ~ 10^-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.

General description

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.

Other Notes

One unit will bind 1.0 μg of d-biotin.

Preparation Note

Affinity purified

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000496586

0000434530

0000316992

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Commercial Plant-Produced Recombinant Protein Products, 15-25 (2014)

Comparison of 211At-PRIT and 211At-RIT of ovarian microtumors in a nude mouse model.

Sofia H L Frost et al.

Cancer biotherapy & radiopharmaceuticals, 28(2), 108-114 (2012-12-13)

Abstract Purpose: Pretargeted radioimmunotherapy (PRIT) against intraperitoneal (i.p.) ovarian microtumors using avidin-conjugated monoclonal antibody MX35 (avidin-MX35) and (211)At-labeled, biotinylated, succinylated poly-l-lysine ((211)At-B-PLsuc) was compared with conventional radioimmunotherapy (RIT) using (211)At-labeled MX35 in a nude mouse model. Mice were inoculated i.p.

Plant molecular farming for the production of valuable proteins - Critical evaluation of achievements and future challenges.

Stefan Schillberg et al.

Journal of plant physiology, 258-259, 153359-153359 (2021-01-19)

Recombinant proteins play an important role in many areas of our lives. For example, recombinant enzymes are used in the food and chemical industries and as high-quality proteins for research, diagnostic and therapeutic applications. The production of recombinant proteins is

Secondary bjerknes forces deform targeted microbubbles.

Tom J A Kokhuis et al.

Ultrasound in medicine & biology, 39(3), 490-506 (2013-01-26)

In this study, we investigated the effect of secondary Bjerknes forces on targeted microbubbles using high-speed optical imaging. We observed that targeted microbubbles attached to an underlying surface and subject to secondary Bjerknes forces deform in the direction of their

Biopharmaceuticals in Plants: Toward the Next Century of Medicine, 121-122 (2009)

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