C1261

Carboxypeptidase A−Agarose

Name: Carboxypeptidase A−Agarose
Brand: SIGMA

ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas

Synonym(s):

Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase

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All Photos(1)

About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.17.1 (BRENDA, IUBMB)

MDL number:

MFCD00130718

Product Name

Carboxypeptidase A−Agarose, ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas

biological source

enzyme from bovine pancreas

form

ammonium sulfate suspension

specific activity

≥6 units/mL packed gel, 25 °C

mol wt

~35,250

matrix

beaded agarose

storage temp.

2-8°C

Quality Level

200

Related Categories

Proteases

Proteins & Enzymes

Biochem/physiol Actions

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.

Carboxypeptidase A is attached covalently to agarose or aldehyde and is effective for immobilization studies.

General description

Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.

Other Notes

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

Physical form

Suspension in 2.0 M (NH₄)₂SO₄, pH 7

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Progressive dry-core-wet-rim hydration trend in a nested-ring topology of protein binding interfaces.

Zhenhua Li et al.

BMC bioinformatics, 13, 51-51 (2012-03-29)

Water is an integral part of protein complexes. It shapes protein binding sites by filling cavities and it bridges local contacts by hydrogen bonds. However, water molecules are usually not included in protein interface models in the past, and few

The zebrafish reveals dependence of the mast cell lineage on Notch signaling in vivo.

Sahar I Da'as et al.

Blood, 119(15), 3585-3594 (2012-03-01)

We used the opportunities afforded by the zebrafish to determine upstream pathways regulating mast cell development in vivo and identify their cellular origin. Colocalization studies demonstrated zebrafish notch receptor expression in cells expressing carboxypeptidase A5 (cpa5), a zebrafish mast cell-specific

Confirmation of a potential biomarker for early-stage pancreatic cancer.

Arthur A Topilow et al.

Cancer biomarkers : section A of Disease markers, 10(1), 27-33 (2012-02-03)

Pancreatic cancer has a dismal prognosis because it is often diagnosed at an advanced stage. Therefore, serological biomarkers are eagerly sought for early detection. The digestive enzyme pro-carboxypeptidase A (PCPA) may be able to fill this role. The purpose of

Stabilization-immobilization of carboxypeptidase A to aldehyde-agarose gels: A practical example in the hydrolysis of casein

Pedroche J, et al.

Enzyme and Microbial Technology, 711-718 null

Identification of two-histidines one-carboxylate binding motifs in proteins amenable to facial coordination to metals.

Beat Amrein et al.

Metallomics : integrated biometal science, 4(4), 379-388 (2012-03-07)

Among natural metalloenzymes, the facial two-histidines one-carboxylate binding motif (FTM) is a widely represented first coordination sphere motif present in the active site of a variety of metalloenzymes. A PDB search revealed a total of 1685 structures bearing such FTMs

View All Related Papers

Protocols

Enzymatic Assay: Carboxypeptidase

Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.