C3865

Anti-N-Cadherin antibody, Mouse monoclonal

Name: Anti-N-Cadherin antibody, Mouse monoclonal
Brand: SIGMA

clone GC-4, purified from hybridoma cell culture

Synonym(s):

Anti-A-CAM, Anti-A-Cell Adhesion Molecule

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All Photos(3)

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164511

Product Name

Anti-N-Cadherin antibody, Mouse monoclonal, clone GC-4, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GC-4, monoclonal

form

buffered aqueous solution

mol wt

antigen 135 kDa

species reactivity

chicken, monkey, mouse, human, rabbit, rat

technique(s)

electron microscopy: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: 10-20 μg/mL using rat cardiac muscle frozen sections
western blot: 10-20 μg/mL using chicken cardiac muscle or COS-7 cell extracts

isotype

IgG1

UniProt accession no.

P19022

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Application

Anti-N-Cadherin antibody is suitable for the following procedures:

electron microscopy
flow cytometry
immunocytochemistry
immunohistochemistry at a concentration of 10-20μg/mL using rat cardiac muscle frozen sections
western blot at a concentration of 10-20μg/mL using chicken cardiac muscle or COS-7 cell extracts

Biochem/physiol Actions

CDH11 gene encodes a type II classical cadherin that mediate calcium-dependent cell-cell adhesion.

Higher expression of CDH2 gene was observed in tumor cells. It is known to induce the invasive and chemoresistant attributes to the cancer cells. N-glycosylation of N-cadherin is associated with the process of tumorigenesis especially in mammary gland, renal system, and ovary. Loss of expression of the gene was observed in adrenocortical carcinoma.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

N-Cadherin is a member of the cadherin family of proteins that mediate cell-cell adhesion in a calcium dependent manner. N-cadherin is a transmembrane glycoprotein that localizes in the cell-cell adherens junction on the plasma membrane. The protein is known to be localized in the cell membrane.

Other Notes

Clone GC-4 is also known as GB-9.

Physical form

0.2 μm-filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

0000522447

0000508935

0000453484

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N-Cadherin-Mediated Signaling Regulates Cell Phenotype for Nucleus Pulposus Cells of the Intervertebral Disc.

Priscilla Y Hwang et al.

Cellular and molecular bioengineering, 8(1), 51-62 (2015-04-08)

Juvenile nucleus pulposus (NP) cells of the intervertebral disc (IVD) are large, vacuolated cells that form cell clusters with strong cell-cell interactions. With maturation and aging, NP cells lose their ability to form these cell clusters, with aging-associated changes in

N-cadherin is Key to Expression of the Nucleus Pulposus Cell Phenotype under Selective Substrate Culture Conditions.

Priscilla Y Hwang et al.

Scientific reports, 6, 28038-28038 (2016-06-14)

Nucleus pulposus (NP) cells of the intervertebral disc are essential for synthesizing extracellular matrix that contributes to disc health and mechanical function. NP cells have a unique morphology and molecular expression pattern derived from their notochordal origin, and reside in

Metformin represses cancer cells via alternate pathways in N-cadherin expressing vs. N-cadherin deficient cells.

Rongbin Ge et al.

Oncotarget, 6(30), 28973-28987 (2015-09-12)

Metformin has emerged as a potential anticancer agent. Here, we demonstrate that metformin plays an anti-tumor role via repressing N-cadherin, independent of AMPK, in wild-type N-cadherin cancer cells. Ectopic-expression of N-cadherin develops metformin-resistant cancer cells, while suppression of N-cadherin sensitizes

Colorectal Cancer Cells Adhere to and Migrate Along the Neurons of the Enteric Nervous System.

Emilie Duchalais et al.

Cellular and molecular gastroenterology and hepatology, 5(1), 31-49 (2017-12-01)

In several types of cancers, tumor cells invade adjacent tissues by migrating along the resident nerves of the tumor microenvironment. This process, called perineural invasion, typically occurs along extrinsic nerves, with Schwann cells providing physical guidance for the tumor cells.

Chemotherapy enriches for an invasive triple-negative breast tumor cell subpopulation expressing a precursor form of N-cadherin on the cell surface.

Nelson ER, et al.

Oncotarget, 7(51), 84030-84030 (2016)

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