C9080

Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse

Name: Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse
Brand: SIGMA

clone V9, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Vimentin

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Product Name

Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse, clone V9, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V9, monoclonal

form

buffered aqueous solution

mol wt

antigen ~58 kDa

species reactivity

pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat

technique(s)

direct immunofluorescence: 1:200 using cultured cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human tonsil
immunohistochemistry (frozen sections): suitable using human tonsil

isotype

IgG1

UniProt accession no.

P08670

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... VIM(7431)
rat ... Vim(81818)

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Application

Monoclonal Anti-Vimentin-Cy3 antibody produced in mouse has been used in:

immunofluorescence and confocal microscopy
cytoskeleton staining
double labeling experiments
immunohistochemical
immunocytochemical localozation

Biochem/physiol Actions

Cy3 Monoclonal Anti-Vimentin reacts with normal and pathological tissue of mesenchymally-derived cells, lens tissue and various cultured cells. It localizes vimentin in fibroblasts, lipocytes, endothelial cells, some lymphoid cells, melanocytes, macrophages, and chondrocytes. The antibody stains tumors derived from these cells, including sarcomas, most lymphomas, melanomas and their metastatic lesions. Co-expression of vimentin with keratin was described in most carcinomas of certain sites (e.g., kidney and thyroid) and certain carcinomas of other sites (e.g., breast).

The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.

Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular space in vitro.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vimentin is expressed in breast myoepithelial cells, osteocytes, Langerhans cells of the skin, Schwann cells and astrocytes.

Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa. Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells.

Immunogen

pig eye lens vimentin.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Store at 2-8 °C. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000475629

0000444756

0000254184

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Development of a constant pressure perfused ex vivo model of the equine larynx.

Sven Otto et al.

PloS one, 16(5), e0251530-e0251530 (2021-05-21)

Distal axonopathy is seen in a broad range of species including equine patients. In horses, this degenerative disorder of the recurrent laryngeal nerve is described as recurrent laryngeal neuropathy (RLN). The dysfunctional innervation of the cricoarytenoideus dorsalis muscle (CAD) leads

Morphology of the myoepithelial cell: immunohistochemical characterization from resting to motile phase

Beha G, et al.

TheScientificWorldJournal, 2012 (2012)

Transdifferentiation of human endothelial progenitors into smooth muscle cells.

HaYeun Ji et al.

Biomaterials, 85, 180-194 (2016-02-14)

Access to smooth muscle cells (SMC) would create opportunities for tissue engineering, drug testing, and disease modeling. Herein we report the direct conversion of human endothelial progenitor cells (EPC) to induced smooth muscle cells (iSMC) by induced expression of MYOCD.

Engineering microenvironment for human cardiac tissue assembly in heart-on-a-chip platform.

Yimu Zhao et al.

Matrix biology : journal of the International Society for Matrix Biology, 85-86, 189-204 (2019-04-15)

Organ-on-a-chip systems have the potential to revolutionize drug screening and disease modeling through the use of human stem cell-derived cardiomyocytes. The predictive power of these tissue models critically depends on the functional assembly and maturation of human cells that are

Coexpression of keratin-and vimentin-type intermediate filaments in human metastatic carcinoma cells

Ramaekers FC, et al.

Proceedings of the National Academy of Sciences of the USA, 80(9), 2618-2622 (1983)

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