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About This Item
UNSPSC Code:
41106514
Biological source:
human colon
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial
biological source
human colon
description
Human colon adenocarcinoma
packaging
tube of 5 μg 87092801-DNA-5UG, pkg of vial of cells 87092801-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Human SW480 cell line has been used to study the antiproliferative effects of F. mughlea Pesmen on cancer cells. It has also been used as an in vitro model to study key cellular processes required for metastasis. The cell line is used to study the effects of the transforming growth factor-β (TGF-β) pathway and heat shock protein 90 (Hsp90) on the growth of cells.
Biochem/physiol Actions
Derived from a grade 3-4 colon adenocarcinoma. The initial cultures contained a mixture of epithelial and bipolar cells, but subsequently epithelial cells predominated. The cells produce CEA.
Human colon adenocarcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 13,14
D13S317: 12
D16S539: 13
D5S818: 13
D7S820: 8
THO1: 8
TPOX: 11
vWA: 16
CSF1PO: 13,14
D13S317: 12
D16S539: 13
D5S818: 13
D7S820: 8
THO1: 8
TPOX: 11
vWA: 16
Preparation Note
L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin; 37°C. Medium change every 4-5 days. CO2 is not required.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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Articles
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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