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About This Item
UNSPSC Code:
41106514
Biological source:
mouse muscle
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Myoblast
biological source
mouse muscle
packaging
tube of 5 μg 91031101-DNA-5UG, pkg of vial of cells 91031101-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Myoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
C2C12 Cell Line has been used to study the the expression of OXT and OXTR (oxytocin and its receptor) genes and proteins and in differentiation studies.
Biochem/physiol Actions
Mouse C3H muscle myoblast
Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.
Preparation Note
Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C. Do not allow cells to reach confluence.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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