D1152

Dulbecco′s Modified Eagle′s Medium - high glucose

Name: Dulbecco′s Modified Eagle′s Medium - high glucose
Brand: SIGMA

HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture

Synonym(s):

DME, DMEM

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

Product Name

Dulbecco′s Modified Eagle′s Medium - high glucose, HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
NaHCO₃: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

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Related Categories

Cell Culture & Analysis

Cell Culture Media & Buffers

Classical Media & Buffers

Dulbecco's Modified Eagle Medium (DMEM)

Molecular Biology & Functional Genomics

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Formulated to contain 17.3 grams of powder per liter of medium.

Supplement with 3.7 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

25G173

0000489938

0000472409

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