D1319

Anti-DAP Kinase 1 antibody produced in rabbit

Name: Anti-DAP Kinase 1 antibody produced in rabbit
Brand: SIGMA

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-DAPK, Anti-DAPK1, Anti-Death associated protein kinase 1

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About This Item

NACRES:

NA.44

UNSPSC Code:

12352203

Product Name

Anti-DAP Kinase 1 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~160 kDa

species reactivity

mouse (predicted), human, rat (predicted)

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 2.5-5.0 μg using extracts of human HeLa cells
western blot: 1-2 μg/mL using whole extracts of human HeLa cells

UniProt accession no.

P53355

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... DAPK1(1612)
mouse ... Dapk1(69635)
rat ... Dapk1(306722)

Application

Anti-DAP Kinase 1 antibody is suitable for use in immunoprecipitation (2.5-5.0 μg using extracts of human HeLa cells) and western blot (1-2 μg/mL using whole extracts of human HeLa cells).

Anti-DAP Kinase 1 antibody produced in rabbit has been used in immunoprecipitation and western blotting.

Biochem/physiol Actions

Anti-DAPK1 antibody is specific for human DAPK1 (approx. 160 kDa). In immunoblotting, detection of the DAPK1 band is specifically inhibited with the immunizing peptide.

Death-associated protein kinase (DAPK) belongs to the serine/threonine kinase family that regulates apoptosis, growth factor signaling and also may be involved in modulating immune responses. DAPK1 mediates cellular functions such as membrane blebbing and autophagy and inhibits the activity of prolyl isomerase

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

DAP-kinase 1 is a Ca²⁺/calmodulin dependent Ser/Thr kinase that associates with microfilaments. The protein is composed of a multidomain structure. It has a subdomain typical of serine/threonine kinases, a Ca²⁺/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. It contains two auto-inhibitory domains, one being Ca²⁺/calmodulin dependent.

Immunogen

synthetic peptide corresponding to amino acids 2-13 of human DAP-kinase 1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Death-associated protein kinase (DAPK) and signal transduction: additional roles beyond cell death.

Yao Lin et al.

The FEBS journal, 277(1), 48-57 (2009-11-03)

Death-associated protein kinase (DAPK) is a stress-regulated protein kinase that mediates a range of processes, including signal-induced cell death and autophagy. Although the kinase domain of DAPK has a range of substrates that mediate its signalling, the additional protein interaction

Iron-loaded transferrin (Tf) is detrimental whereas iron-free Tf confers protection against brain ischemia by modifying blood Tf saturation and subsequent neuronal damage

DeGregorio-RN, et al.

Redox Biology, 143-158 (2018)

DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity

Cohen O, et al.

The Embo Journal, 16(5), 998-1008 (1997)

DAPK1 mediates the G1 phase arrest in human nasopharyngeal carcinoma cells induced by grifolin, a potential antitumor natural product

Luo XJ, et al.

European Journal of Pharmacology, 670(2-3), 427-434 (2011)

Grifolin, a potent antitumour natural product upregulates death-associated protein kinase 1 DAPK1 via p53 in nasopharyngeal carcinoma cells