D8764
Deoxyribonuclease II from bovine spleen
Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein
Synonym(s):
DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-801-8
MDL number:
Specific activity:
≥1,000 units/mg protein
Biological source:
bovine spleen
Concentration:
≥50% protein
biological source
bovine spleen
type
Type V
form
essentially salt-free, lyophilized powder
specific activity
≥1,000 units/mg protein
mol wt
38 kDa
concentration
≥50% protein
technique(s)
DNA extraction: suitable, tissue culture: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
foreign activity
RNase ≤0.1%
storage temp.
−20°C
Quality Level
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Application
Deoxyribonuclease II from bovine spleen has been used:
- as a component of digestion mix to isolate immune cells from colon samples
- to digest retinal explants for single-cell suspension preparation
- for isolation of stromal cells from human colon tissue
- in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart
Biochem/physiol Actions
Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.
Other Notes
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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K Nitta et al.
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 114(2), 119-128 (1994-02-01)
Deoxyribonucleases from eggs and the liver of Xenopus laevis were partially purified by DEAE-cellulose and heparin-Sepharose affinity column chromatographies. The fractions having egg and liver DNase activities were eluted on high performance liquid chromatography through TSK gel G3000SW at the
S Ingebrandt et al.
Biosensors & bioelectronics, 16(7-8), 565-570 (2001-09-07)
An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described
Dopamine D2 receptor stimulation differentially affects voltage-activated calcium channels in rat pituitary melanotropic cells.
Keja J A, et al.
The Journal of Physiology, 450(1), 409-435 (1992)
Therese Ostberg et al.
Arthritis and rheumatism, 62(10), 2963-2972 (2010-06-10)
High mobility group box chromosomal protein 1 (HMGB-1) is a DNA binding nuclear protein that can be released from dying cells and activated myeloid cells. Extracellularly, HMGB-1 promotes inflammation. Clinical and experimental studies demonstrate that HMGB-1 is a pathogenic factor
STUDIES ON ACID DEOXYRIBONUCLEASE. II. ISOLATION AND CHARACTERIZATION OF SPLEEN-ACID DEOXYRIBONUCLEASE.
G BERNARDI et al.
Biochemistry, 3, 1419-1426 (1964-10-01)
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