E2520
Anti-Epidermal Growth Factor antibody, Mouse monoclonal
clone EGF-10, purified from hybridoma cell culture
Synonym(s):
Anti-CEGF
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About This Item
Product Name
Anti-Epidermal Growth Factor antibody, Mouse monoclonal, clone EGF-10, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
EGF-10, monoclonal
form
buffered aqueous solution
mol wt
antigen 6 kDa
species reactivity
human
technique(s)
dot blot: 0.1 μg/mL using recombinant human EGF
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human tissue
indirect ELISA: suitable
microarray: suitable
neutralization: suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... EGF(1950)
Application
Monoclonal Anti-Epidermal Growth Factor antibody has been used in dot-blot, radioimmunoassay, enzyme linked immunosorbent assay (ELISA), immunohistology, immunoblotting, immunostaining.
Monoclonal Anti-Epidermal Growth Factor antibody produced in mouse was used to study rat immune cells by flow cytometry to detect the presence or absence of hormone and hormone-like molecules in response to EGF treatment.
Biochem/physiol Actions
EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.
Epidermal growth factor (EGF) is a potent growth-promoting factor for a variety of tissue cells in vivo and in vitro. EGF binding induces phosphatidylinositide hydrolysis and generation of diacylglycerol (DAG). Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis and synthesis of DNA, RNA and proteins. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells. The biological role of EGF includes the inhibition of gastric acid secretion, support of growth and differentiation during fetal development, neuromodulation in the central nervous system and stimulation of epidermal growth and keratinization.
Specifically reacts with both native and recombinant human EGF. The antibody does not cross-react with mouse EGF.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Epidermal Growth Factor (EGF) (mouse IgG1 isotype) is derived from the EGF-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Epidermal growth factor (EGF) is a single chain polypeptide hormone (6 kDa). EGF protein exists largely in a β-sheet conformation, with a C-terminal hairpin that may be involved in receptor binding. Human EGF is found in body fluids such as urine, milk, saliva, sweat, and seminal fluid and has its highest concentration in α-granules of blood platelets.
Immunogen
recombinant human EGF
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Marie McIlroy et al.
Cardiovascular research, 69(1), 207-217 (2005-09-17)
Pericytes, located in close proximity to the underlying endothelium, form an integral component of the microvasculature. These cells are intimately involved in angiogenesis, which is of fundamental importance in many physiological and pathological processes. We evaluated the influence of pericyte-conditioned
Effects of triple treatment with octreotide, galanin and serotonin on a human pancreas cancer cell line in xenografts
El-Salhy M et al.
Histology and Histopathology, 14(6), 3550-3558 (2005)
Epidermal growth factor (EGF) in the human placental infection with Trypanosoma cruzi
Lin S, et al.
Placenta, 25(4), 283-286 (2004)
ErbB3 is involved in activation of phosphatidylinositol 3-kinase by epidermal growth factor.
Soltoff S P, et al.
Molecular and Cellular Biology, 14(6), 3550-3558 (1994)
Clémence Dubois et al.
Journal of clinical medicine, 8(3) (2019-03-25)
Triple-negative breast cancers are particularly aggressive. In vitro cultures are one of the major pathways for developing anticancer strategies. The effectiveness and reproducibility of the drug screenings depend largely on the homogeneity of culture media. In order to optimize the
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