E2884

Esterase from porcine liver

Name: Esterase from porcine liver
Brand: SIGMA

ammonium sulfate suspension, ≥150 units/mg protein (biuret)

Synonym(s):

Carboxyl esterase, Carboxylic-ester hydrolase, PLE

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About This Item

CAS Number:

9016-18-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.1.1.1 (BRENDA, IUBMB)

MDL number:

MFCD00131030

Product Name

Esterase from porcine liver, ammonium sulfate suspension, ≥150 units/mg protein (biuret)

biological source

Porcine liver

form

ammonium sulfate suspension

specific activity

≥150 units/mg protein (biuret)

mol wt

168 kDa

storage temp.

2-8°C

Quality Level

300

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Application

Esterase from porcine liver has been used to unmask the fluorescence in ultraviolet-visible spectroscopy.

Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.

Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.

The enzyme from Sigma has been used to develop an alternative gel-based protocol for screening for bead-bound catalytic activity in aqueous media. The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).

Biochem/physiol Actions

Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.

Esterase enzyme of bacteria and fungi seems to have several industrial applications. It is capable of catalyzing the hydrolysis and synthesis of ester bonds.

Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.

General description

Esterase, also known as carboxyl ester hydrolase is a member of the hydrolase superfamily of enzymes.

Other Notes

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, pH 8

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Synthesis of coumarin-based, esterase-sensitive cyclic prodrugs of opioid peptides with enhanced membrane permeability and enzymatic stability.

B Wang et al.

Methods in molecular medicine, 23, 71-85 (1999-01-01)

With the discovery of an increasing number of biologically active peptides and peptide mimetics (1-3), there is a pressing need for the development of strategies to deliver these biologically active compounds to the desired site of action. The preceding two

Inhibition of lipoprotein-associated phospholipase A2 reduces complex coronary atherosclerotic plaque development.

Robert L Wilensky et al.

Nature medicine, 14(10), 1059-1066 (2008-09-23)

Increased lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) activity is associated with increased risk of cardiac events, but it is not known whether Lp-PLA(2) is a causative agent. Here we show that selective inhibition of Lp-PLA(2) with darapladib reduced development of advanced coronary

An unexpected plasma cholinesterase activity rebound after challenge with a high dose of the nerve agent VX.

F Dorandeu et al.

Toxicology, 248(2-3), 151-157 (2008-05-03)

Organophosphorus chemical warfare agents (nerve agents) are to be feared in military operations as well as in terrorist attacks. Among them, VX (O-ethyl-S-[2-(diisopropylamino)ethyl] methylphosphonothioate) is a low volatility liquid that represents a percutaneous as well as an inhalation hazard if

Fluorogenic affinity label for the facile, rapid imaging of proteins in live cells

Watkins RW, et al.

Organic & Biomolecular Chemistry, 7(19), 3969-3975 (2019)

Synthesis and properties of a new water-soluble prodrug of the adenosine A 2A receptor antagonist MSX-2.

Karl Vollmann et al.

Molecules (Basel, Switzerland), 13(2), 348-359 (2008-02-29)

The compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4) was synthesized as an amino acid ester prodrug of the adenosine A2A receptor antagonist MSX-2. It was found to be stable in artificial gastric acid, but readily cleaved by pig liver esterase.

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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocols

Enzymatic Assay of Esterase

Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.