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Merck

E5391

Ethylenediaminetetraacetic acid tetrasodium salt hydrate

≥99.0%

Synonym(s):

EDTA tetrasodium salt, Edathamil, Tetrasodium ethylenediaminetetraacetate hydrate

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About This Item

Linear Formula:
(NaOOCCH2)2NCH2CH2N(CH2COONa)2 · xH2O
CAS Number:
194491-31-1
Molecular Weight:
380.17 (anhydrous basis)
UNSPSC Code:
12352204
NACRES:
NA.83
PubChem Substance ID:
24894613
EC Number:
200-573-9
Beilstein/REAXYS Number:
3861753
MDL number:
MFCD00150025

Product Name

Ethylenediaminetetraacetic acid tetrasodium salt hydrate, ≥99.0%

InChI key

GXFFSKMBMYHTAE-UHFFFAOYSA-J

InChI

1S/C10H16N2O8.4Na.H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;;1H2/q;4*+1;/p-4

SMILES string

O.[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC([O-])=O

biological source

synthetic (organic)

assay

≥99.0%

form

powder

technique(s)

ligand binding assay: suitable

impurities

Insoluble matter, passes filter test

pH

11.0-12.0 (0.1 M in H2O)

solubility

H2O: 0.1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.01%

cation traces

Al: ≤0.001%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: <0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorption

≤0.06 at 280 in H2O at 0.1 M
≤1.5 at 260 in H2O at 0.1 M

application(s)

sample preparation

storage temp.

room temp

Quality Level

200

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Application

EDTA is an inhibitor of metalloproteases, at effective concentrations of 1-10 mM. EDTA acts as a chelator of the zinc ion in the active site of metalloproteases, and can also inhibit other metal ion-dependent proteases such as calcium-dependent cysteine proteases. For use as an anticoagulant, disodium or tripotassium salts of EDTA are most commonly used. The optimal concentration is 1.5 mg per ml of blood.
Used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors.

Preparation Note

Soluble in water at room temperature up to 1.45 M. The pH of this solution will be approximately 10 to 11 and will be rather viscous.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP1262
0000507746
0000507746
0000507083
0000507083

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