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Merck

F0392

Fetal Bovine Serum

USA origin, Dialyzed by ultrafiltration against 0.15 M NaCl, sterile-filtered, suitable for cell culture

Synonym(s):

Fetal Bovine Sera, Fetal Calf Sera, FBS, FCS, sera, serum

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160409
NACRES:
NA.71
MDL number:
biological source:
bovine fetus
origin:
USA origin
sterility:
sterile-filtered
form:
liquid
shipped in:
dry ice

Product Name

Fetal Bovine Serum, USA origin, Dialyzed by ultrafiltration against 0.15 M NaCl, sterile-filtered, suitable for cell culture

biological source

bovine fetus

sterility

sterile-filtered

product line

FBS Select

form

liquid

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Quality Level

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Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Application

Fetal Bovine Serum has been used as a supplement for various cell culture techniques as it provides many non-defined growth promoting and survival enhancing factor to cells in culture.

Biochem/physiol Actions

Fetal Bovine Serum (FBS) is a constituent of animal cell culture media. It contains hormones and growth factors. FBS promotes in vitro cell culture, by providing binding proteins, lipids, minerals, attachment and spreading factors.

Preparation Note

10,000 Molecular Weight Cut-off

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Direct interactions with both p27 and Cdk2 regulate Spy1-mediated proliferation in vivo and in vitro
Al Sorkhy M, et al.
Cell Cycle, 15(1), 128-136 (2016)
The use of fetal bovine serum: ethical or scientific problem?
Jochems CEA, et al.
Alternatives to Laboratory Animals : ATLA, 30(2), 219-227 (2002)
Stimulatory effect of luteinizing hormone, insulin-like growth factor-1, and epidermal growth factor on vascular endothelial growth factor production in cultured bubaline luteal cells
Chouhan VS, et al.
Theriogenology, 84(7), 1185-1196 (2015)
Blocking anaplerotic entry of glutamine into the TCA cycle sensitizes K-Ras mutant cancer cells to cytotoxic drugs
Saqcena M, et al.
Oncogene, 34(20), 2672-2672 (2015)
Liver Tissue Engineering Based on Poly (ethylen Glycol) Hydrogels (2010)

Articles

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Protocols

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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