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F2555

Monoclonal ANTI-FLAG® antibody produced in rabbit

clone SIG1-25, ascites fluid

Synonym(s):

Anti-ddddk, Anti-dykddddk

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
SIG1-25, monoclonal
Application:
ELISA (i), ICC, WB
Citations:
133
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biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SIG1-25, monoclonal

technique(s)

immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100, indirect ELISA: suitable, western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

General description

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in association with other tags. The small size of the FLAG tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function. The N-terminal FLAG® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage catalyzed by Cu2+ ions of the C-terminal FLAG® peptide from a fusion protein has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG® peptide is found in both rat and mouse Mg2+ dependent protein b-phosphatase, as well as in the human and bovine enzyme. This rabbit ANTI-FLAG® antibody is produced from the hybridoma SIG1-25. The hybridoma is derived by the fusion of myeloma cells and splenocytes from rabbits immunized with the FLAG® peptide sequence conjugated to KLH.

Immunogen

FLAG peptide sequence DYKDDDDK

Application

Monoclonal ANTI-FLAG® antibody produced in rabbit has been used in:

  • immunofluorescence
  • western blot analysis
  • immunocytochemistry
  • indirect ELISA

Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

This monoclonal antibody recognizes the FLAG sequence at the N-terminus

Physical form

Provided as ascites fluid containing 15 mM sodium azide as a preservative.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000435608
0000435608
0000368976
0000223737
0000223737

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Articles

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Analysis of heavy metals and metals in cannabis and hemp containing personal care products and cosmetics include sample preparation by grinding, preparation of calibration solutions for a standard addition approach, a microwave digestion protocol and subsequent ICP-MS analysis.

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Datasheet - F2555
Recombinant lentivirus with enhanced expression of caudal-related homeobox protein 2 inhibits human colorectal cancer cell proliferation in vitro
He S, et al.
Molecular Medicine Reports, 12(2), 1838-1844 (2015)
Reduction of NANOG mediates the inhibitory effect of aspirin on tumor growth and stemness in colorectal cancer
Wang H, et al.
Cellular Physiology and Biochemistry, 44(3), 1051-1063 (2017)
Jennifer J Banerjee et al.
eLife, 6 (2017-07-01)
Polarity is a shared feature of most cells. In epithelia, apical-basal polarity often coexists, and sometimes intersects with planar cell polarity (PCP), which orients cells in the epithelial plane. From a limited set of core building blocks (e.g. the Par
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