F3133

Fibroblast Growth Factor from bovine pituitary

Name: Fibroblast Growth Factor from bovine pituitary
Brand: SIGMA

lyophilized, suitable for cell culture

Synonym(s):

FGF

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About This Item

CAS Number:

62031-54-3

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00166724

Product Name

Fibroblast Growth Factor from bovine pituitary, suitable for cell culture

biological source

bovine pituitary glands

form

lyophilized powder

potency

1-100 ng/mL

mol wt

16.4 kDa

packaging

pkg of 10 and 5X10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white to off-white

storage temp.

−20°C

Quality Level

200

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Analysis Note

Although the major mitogenic component of this product is FGF2, the activity per unit weight of protein is less than that of purified FGF2.

The biological activity is measured using a 3-day MTT assay.

Application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.

Fibroblast Growth Factor from bovine pituitary has been used in in vitro testicular cell culture. It has also been used as a component of myogenic culture medium.

Biochem/physiol Actions

Fibroblast growth factors (FGF) actively participate in cellular responses including embryogenesis and organogenesis. It specifically controls cell proliferation, differentiation and migration. FGF is known to regulate nervous system functions, tissue repair mechanism and wound healing. It also controls angiogenesis of tumor cells. Abnormal FGF mediated signaling induces tumor angiogenesis, by stimulating cancer cell proliferation and survival. FGFs carries out its function by mediating through four receptor tyrosine kinases : FGF-receptors FGFR1, FGFR2, FGFR3 and FGFR4.

General description

Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34kDa and they possess a conserved sequence of 120 amino acids.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.1 mg/ml BSA.

Preparation Note

Purified glands by a modification of the method of Gospodarowicz.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Modulation of osteogenic, adipogenic and myogenic differentiation of mesenchymal stem cells by submicron grooved topography

Wang PY, et al.

Journal of Materials Science. Materials in Medicine, 23(12), 3015-3028 (2012)

Cellular signaling by fibroblast growth factor receptors

Eswarakumar VP, et al.

Cytokine & Growth Factor Reviews, 16(2), 139-149 (2005)

Fibroblast growth factor signalling: from development to cancer

Turner N and Grose R

Nature Reviews. Cancer, 10(2), 116-116 (2010)

Identification, culture, and characterization of germline stem cell-like cells in chicken testes

Jung JG, et al.

Biology of Reproduction, 76(1), 173-182 (2007)

Characterization and In Vitro Differentiation of Korean Ring-Necked Pheasant (Phasianus colchicus) Male Germ Cells

Jeong DK, et al.

Journal of Embryology transfer , 29(4), 351-359 (2014)

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