F8424
Fibroblast Growth Factor-4 human
FGF-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
hFGF-4, FGF-4, K-FGF
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About This Item
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
monomer 19 kDa ((177 aa))
Impurities:
<1 EU/μg (LAL test)
biological source
human
recombinant
expressed in E. coli
form
lyophilized powder
potency
≤10.0 ng/mL
quality
endotoxin tested
packaging
pkg of 25 and 100 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... FGF4(2249), FGF4(2249)
assay
≥95% (SDS-PAGE)
mol wt
monomer 19 kDa ((177 aa))
impurities
<1 EU/μg (LAL test)
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General description
Fibroblast Growth Factor-4 (FGF4) is a 19 kDa protein, belonging to the FGF family of proteins. Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34 kDa and they possess a conserved sequence of 120 amino acids. The FGF4 gene is mapped to human chromosome 11q13.3.
Application
Fibroblast Growth Factor-4 human has been used:
- To maintain trophectoderm stem cells.
- In neurogenic differentiation.
- In hepatocyte differentiation.
Biochem/physiol Actions
Fibroblast growth factor-4 (FGF-4) is a 19 kDa protein identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development.
Fibroblast growth factor-4 (FGF-4) is identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development. FGF-4 participates in cell differentiation and cell migration.
Physical form
Lyophilized from a 0.2 µm filtered solution containing 20 mM Sodium Phosphate, 0.5 M Sodium Chloride, pH 7.5
Preparation Note
Reconstitution: Sterile water at 0.1 mg/mL
Analysis Note
The biological activity is measured by its ability to stimulate NR6R-3T3 cell proliferation.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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The oncoprotein HBXIP up-regulates FGF4 through activating transcriptional factor Sp1 to promote the migration of breast cancer cells
Shi H, et al.
Biochemical and biophysical research communications, 471(1), 89-94 (2016)
Riley McMahon et al.
Frontiers in cell and developmental biology, 9, 777652-777652 (2022-02-19)
The specification of anterior head tissue in the late gastrulation mouse embryo relies on signaling cues from the visceral endoderm and anterior mesendoderm (AME). Genetic loss-of-function studies have pinpointed a critical requirement of LIM homeobox 1 (LHX1) transcription factor in
Fibroblast growth factor signalling: from development to cancer
Turner N and Grose R
Nature Reviews. Cancer, 10(2), 116-116 (2010)
Endometrial regenerative cells: a novel stem cell population
Meng X, et al.
Journal of Translational Medicine, 5(1), 57-57 (2007)
Cellular signaling by fibroblast growth factor receptors
Eswarakumar VP, et al.
Cytokine & Growth Factor Reviews, 16(2), 139-149 (2005)
Articles
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