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Merck

G5894

Glutaminase from Escherichia coli

Grade VII, lyophilized powder, 500-1,500 units/mg protein

Synonym(s):

L-Glutamine amidohydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
500-1,500 units/mg protein
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type

Grade VII

Quality Level

form

lyophilized powder

specific activity

500-1,500 units/mg protein

composition

Protein, ~10% Lowry

storage temp.

−20°C

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Biochem/physiol Actions

Glutaminase catalyzes the conversion of glutamine to glutamate.

Physical form

Lyophilized powder containing stabilizer and potassium succinate

Other Notes

Chromatographically purified from Grade V
One unit will deaminate 1.0 μmole of L-glutamine per min at pH 4.9 at 37 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Felix List et al.
Chemistry & biology, 19(12), 1589-1599 (2012-12-25)
Nitrogen is incorporated into various metabolites by multifunctional glutamine amidotransferases via reactive ammonia generated by glutaminase hydrolysis of glutamine. Although this process is generally tightly regulated by subsequent synthase activity, little is known about how the glutaminase is inhibited in
Catherine Qiurong Pan et al.
FEBS letters, 586(17), 2674-2691 (2012-06-20)
The BNIP-2 and Cdc42GAP Homology (BCH) domains constitute a new and expanding family of highly conserved scaffold protein domains that regulate Rho, Ras and MAPK signaling, leading to cell growth, apoptosis, morphogenesis, migration and differentiation. Such versatility is achieved via
Kristin F Wilson et al.
Trends in molecular medicine, 19(2), 74-82 (2012-12-12)
Recently, the small molecule 968 was found to block the Rho GTPase-dependent growth of cancer cells in cell culture and mouse xenografts, and when the target of 968 was found to be the mitochondrial enzyme glutaminase (GLS1), it revealed a
N V Zharkova et al.
Biomeditsinskaia khimiia, 58(2), 220-223 (2012-06-26)
Acute alcohol intoxication in rats with alloxan diabetes is accompanied by the increase of urea and uric acid and by the decrease in free fatty acids in serum. In the liver of experimental animals the increase of activity of glutamate
Krupa Shukla et al.
Journal of medicinal chemistry, 55(23), 10551-10563 (2012-11-16)
Bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl)ethyl sulfide (BPTES) is a potent and selective allosteric inhibitor of kidney-type glutaminase (GLS) that has served as a molecular probe to determine the therapeutic potential of GLS inhibition. In an attempt to identify more potent GLS inhibitors with improved

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