H3631

Hyaluronidase from bovine testes

Name: Hyaluronidase from bovine testes
Brand: SIGMA

Type VI-S, lyophilized powder, 3,000-15,000 units/mg solid

Synonym(s):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

CAS Number:

37326-33-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

253-464-3

MDL number:

MFCD00131351

EC Number:

3.2.1.35 (BRENDA, IUBMB)

Product Name

Hyaluronidase from bovine testes, Type VI-S, lyophilized powder, 3,000-15,000 units/mg solid

biological source

bovine testis

conjugate

conjugate (Glucosaminoglycan)

type

Type VI-S

form

lyophilized powder

specific activity

3,000-15,000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

UniProt accession no.

Q5E985
Q8SQG8

relevant disease(s)

cancer

storage temp.

−20°C

Quality Level

200

Gene Information

cow ... HYAL1(515397), HYAL2(281838)

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Related Categories

Glycobiology Profiling Tools

Proteins & Enzymes

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Preparation Note

Chromatographically purified

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000484419

0000484418

0000457364

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Single-cell glycomics analysis by CyTOF-Lec reveals glycan features defining cells differentially susceptible to HIV.

Tongcui Ma et al.

eLife, 11 (2022-07-06)

High-parameter single-cell phenotyping has enabled in-depth classification and interrogation of immune cells, but to date has not allowed for glycan characterization. Here, we develop CyTOF-Lec as an approach to simultaneously characterize many protein and glycan features of human immune cells

Effect of hyaluronic acid on urine nerve growth factor in cyclophosphamide-induced cystitis.

Dong-Ru Ho et al.

International journal of urology : official journal of the Japanese Urological Association, 18(7), 525-531 (2011-05-25)

To investigate how hyaluronic acid (HA) affects nerve growth factor (NGF) production and bladder overactivity in a cyclophosphamide (CYP)-induced cystitis rat model. Female Sprague-Dawley rats received three intermittent intraperitoneal injections of CYP (75 mg/kg) or saline. Before or after CYP injection

HIV efficiently infects T cells from the endometrium and remodels them to promote systemic viral spread.

Tongcui Ma et al.

eLife, 9 (2020-05-27)

The female reproductive tract (FRT) is the most common site of infection during HIV transmission to women, but viral remodeling complicates characterization of cells targeted for infection. Here, we report extensive phenotypic analyses of HIV-infected endometrial cells by CyTOF, and

Shared decision making, thrombotic thrombocytopenic purpura, and caplacizumab.

Senthil Sukumar et al.

American journal of hematology, 95(4), E76-E77 (2020-01-03)

The cancer glycocalyx mediates intravascular adhesion and extravasation during metastatic dissemination.

Giovanni S Offeddu et al.

Communications biology, 4(1), 255-255 (2021-02-28)

The glycocalyx on tumor cells has been recently identified as an important driver for cancer progression, possibly providing critical opportunities for treatment. Metastasis, in particular, is often the limiting step in the survival to cancer, yet our understanding of how

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Articles

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.