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Merck

H6533

Anti-HA−Peroxidase antibody, Mouse monoclonal

clone HA-7, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
MFCD00803873

Product Name

Anti-HA−Peroxidase antibody, Mouse monoclonal, clone HA-7, purified from hybridoma cell culture

biological source

mouse

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HA-7, monoclonal

form

lyophilized powder

packaging

vial of 0.5 mL

concentration

5-11 mg/mL

technique(s)

western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent

storage temp.

2-8°C

Quality Level

200

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Application

Monoclonal Anti-HA-Peroxidase antibody produced in mouse has been used:
  • in immunopreciptation and immunostaining of human member 9 of solute carrier family 38 SLC38A9 transfected in HeLa cells
  • in immunoblot detection in transfected A375 cells
  • is suitable for immunoblotting of nucleosome assembly protein NAP1L1

Biochem/physiol Actions

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

General description

Influenza A virus, a RNA virus, encodes two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). HA stalk domain based epitope region antibody is effective in neutralizing divergent influenza virus strains.
The mouse monoclonal Anti-HA-Peroxidase antibody recognizes native and denatured-reduced forms of the HA-tagged proteins. It also reacts with the N- or C-terminal HA-tagged fusion proteins expressed in E. coli and mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Reconstitute in .5mL of distilled water.

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pictograms

Exclamation mark
GHS07

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Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000377759
0000377759
0000211365
0000211365
0000125303

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?

    Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Luo M, et al.
Cellular Signalling, 42, 30-43 (2018)
Hepatitis C virus NS5A targets nucleosome assembly protein NAP1L1 to control the innate cellular response
Cevik RE, et al.
Journal of virology, 91(18), e00880-e00817 (2017)
Ernest C So et al.
The Journal of biological chemistry, 291(11), 5832-5843 (2016-01-13)
Legionella pneumophila, the causative agent of Legionnaire's disease, uses its type IV secretion system to translocate over 300 effector proteins into host cells. These effectors subvert host cell signaling pathways to ensure bacterial proliferation. Despite their importance for pathogenesis, the
A broadly reactive human anti-hemagglutinin stem monoclonal antibody that inhibits influenza a virus particle release
Yamayoshi S, et al.
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Influenza virus hemagglutinin stalk-based antibodies and vaccines
Krammer F and Palese P
Current Opinion in Virology, 3(5), 521-530 (2013)
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