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Merck

HT501640

Formalin solution, neutral buffered, 10%

histological tissue fixative

Synonym(s):

10% NBF, 10% Neutral Buffered Formalin, Formaldehyde, Methanal

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About This Item

UNSPSC Code:
12352114
PubChem Substance ID:
NACRES:
NA.47
MDL number:

Product Name

Formalin solution, neutral buffered, 10%, histological tissue fixative

SMILES string

[O-]#[C+H2]

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

concentration

10% formalin (approx. 4% formaldehyde)

color

colorless

pH

6.90-7.10

density

1.080 g/cm3

application(s)

hematology
histology

storage temp.

room temp

Quality Level

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Application

Formalin solution (neutral buffered, 10%) has been popularly used as a general-purpose histological fixative to fix tissue samples.
  • It helps to preserve tissue samples by preventing decay and maintaining their molecular integrity.
  • It effectively preserves the cellular and tissue morphology, maintaining the structural integrity required for microscopic examination.
  • It is used for routine histological staining, providing clear and detailed images of cellular structures.
Popular general purpose histological fixative.

Biochem/physiol Actions

Formaldehyde is both a non-coagulant and an additive fixative. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins.

Features and Benefits

  • 10% NBF is well-suited for large throughput laboratories.
  • It requires a relatively short period of fixation.
  • It can be used for the long-term storage of tissue.

General description

10% Neutral Buffered Formalin is the most used fixative throughout the world for light microscopy. Formalin solution is the commercially obtained solution containing 37% w/w (40% w/v) of formaldehyde in water. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.

Packaging

Item is packaged in a 20 L Cubitainer consisting of a plastic bladder inside of a cardboard box with an attached spigot.

Physical form

Neutral buffered solution

pictograms

Health hazardExclamation mark

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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

wgk

WGK 3

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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