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Merck

K1377

Kanamycin sulfate from Streptomyces kanamyceticus

powder, BioReagent, suitable for cell culture, suitable for plant cell culture

Synonym(s):

Kanamycin A, Kanamycin sulfate salt

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About This Item

Empirical Formula (Hill Notation):
C18H36N4O11 · H2O4S
CAS Number:
25389-94-0
Molecular Weight:
582.58
NACRES:
NA.76
PubChem Substance ID:
24896204
UNSPSC Code:
51281654
EC Number:
246-933-9
MDL number:
MFCD00070253

Product Name

Kanamycin sulfate from Streptomyces kanamyceticus, powder, BioReagent, suitable for cell culture, suitable for plant cell culture

InChI key

OOYGSFOGFJDDHP-KMCOLRRFSA-N

InChI

1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1

SMILES string

OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

biological source

Streptomyces kanamyceticus

product line

BioReagent

form

powder

potency

≥750 μg per mg (dry basis)

technique(s)

cell culture | mammalian: suitable
cell culture | plant: suitable

color

white to off-white

solubility

H2O: 10-50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 5 days.)

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycobacteria
mycoplasma

application(s)

agriculture

mode of action

protein synthesis | interferes

Quality Level

200

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Application

Kanamycin sulfate from Streptomyces kanamyceticus has been used in tissue culture media.

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Disclaimer

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

General description

Chemical structure: aminoglycoside
Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100μg/mL.

Preparation Note

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H360D

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000488865
0000488865
0000494666
0000494666
0000462292

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Moxalactam as a counter-selection antibiotic for Agrobacteriummediated
transformation and its positive effects on Theobroma cacao
somatic embryogenesis
Gabriela Antu'nez de Mayolo
Plant Science, 164, 607-615 (2003)
Samaneh Mosallatpour et al.
Scientific reports, 9(1), 19062-19062 (2019-12-15)
L-glutaminase importance to use in the food industry and medicine has attracted much attention. Enzymes stability has always been a challenge while working with them. We heterologously expressed and characterized a novel stable L-glutaminase from an extremophile bacterium (Cohnella sp.
Riccardo Di Mambro et al.
Current biology : CB, 29(7), 1199-1205 (2019-03-19)
Plant developmental plasticity relies on the activities of meristems, regions where stem cells continuously produce new cells [1]. The lateral root cap (LRC) is the outermost tissue of the root meristem [1], and it is known to play an important
Mitsutaka Kitano et al.
Antimicrobial agents and chemotherapy, 58(8), 4795-4803 (2014-06-11)
Highly pathogenic avian influenza A (H5N1) viruses cause severe and often fatal disease in humans. We evaluated the efficacy of repeated intravenous dosing of the neuraminidase inhibitor peramivir against highly pathogenic avian influenza virus A/Vietnam/UT3040/2004 (H5N1) infection in cynomolgus macaques.
Atef Allam et al.
Journal of immunology (Baltimore, Md. : 1950), 193(2), 871-878 (2014-06-11)
The role of the TNF family member CD70 in adaptive T cell responses has been intensively studied, but its function in innate responses is still under investigation. In this study, we show that CD70 inhibits the early innate response to
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Product Information Sheet - K1377

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