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Merck

L4646

Lipids Cholesterol Rich from adult bovine serum

liquid, sterile-filtered, BioReagent, suitable for cell culture, suitable for insect cell culture

Synonym(s):

Bovine Serum Lipids, Cholesterol Rich Lipids, Lipids from Bovine

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352211

Key Documents

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sterility

sterile-filtered

product line

BioReagent

form

liquid

composition

Cholesterol, 9.0-11.0 g/L , Protein, 15.0-25.0 g/L

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

impurities

Adventitious viral agents, none detected (9 CFR; 113.53), IgG, none detected (RID), Microbial content, none detected (USP XXII), Mycoplasma, none detected (Barile method), ≤6.0 EU/mg cholesterol endotoxin

storage temp.

2-8°C

Quality Level

200

Related Categories

General description

Cholesterol is a lipid molecule, found integrated to the cell membrane.

Application

Lipids Cholesterol Rich from adult bovine serum has been used as one of the lipid component to generate erythroblasts ex vivo. It has also been used as a standard to determine muscle cholesterol level, spectrophotometrically.

Biochem/physiol Actions

Cholesterol significantly affects the property of phospholipid membrane such as fluidity and membrane protein function. It exhibits a condensing effect towards phospholipids. Cholesterol-phospholipid interaction influences physiological properties of a lipid bilayer. Cholesterol serves as a precursor of bile acids, steroid hormones and vitamin D. Uncontrolled level of plasma cholesterol might lead to the risk of cardiovascular disorder.

Other Notes

Aqueous lipoprotein solution

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000433583
0000433583
0000376398
0000333081
0000376398

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Fatty acid profile, cholesterol and oxidative status in broiler chicken breast muscle fed different dietary oil sources and calcium levels
Abdulla NR, et al.
South African Journal of Animal Science, 45(2), 153-163 (2015)
The condensing effect of cholesterol in lipid bilayers
Hung WC, et al.
Biophysical Journal, 92(11), 3960-3967 (2007)
Effect of cholesterol on the structure of a phospholipid bilayer
de Meyer F and Smit B
Proceedings of the National Academy of Sciences of the USA, 106(10), 3654-3658 (2009)
Paoletti R. and Kritchevsky D.
Advances in Lipid Research, 8, 1-1 (2014)
Humanized culture medium for clinical expansion of human erythroblasts
Migliaccio G, et al.
Cell Transplantation, 19(4), 453-469 (2010)
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