L6632
Lipoxidase from Glycine max (soybean)
Type V, ammonium sulfate suspension, 500,000-1,000,000 units/mg protein
Synonym(s):
Linoleate:oxygen oxidoreductase, Lipoxygenase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-853-1
MDL number:
EC Number:
Specific activity:
500,000-1,000,000 units/mg protein
type
Type V
Quality Level
form
ammonium sulfate suspension
specific activity
500,000-1,000,000 units/mg protein
mol wt
94 kDa
storage temp.
2-8°C
Application
Lipoxidase, or lipoxygenase, from Glycine max (soybean) has been used for the modification of low density lipoprotein, isolated from human plasma.
The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).
Biochem/physiol Actions
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Physical form
Suspension in 2.3 M (NH4)2SO4 solution, pH approx. 6.0
Preparation Note
Prepared by ion exchange chromatography and hydrophobic interaction chromatography.
Analysis Note
Protein determined by biuret.
Other Notes
One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
P Harduin et al.
Journal of lipid research, 36(5), 919-930 (1995-05-01)
We studied the effect of in vitro moderate oxidation on low density lipoprotein (LDL) conformation and metabolism. LDL was modified with either copper ions or phospholipase A2 plus lipoxygenase and, in both cases, mild oxidative conditions were used. The resulting
Nisreen Faizo et al.
Foods (Basel, Switzerland), 10(2) (2021-02-07)
Lipid peroxides (LOOHs) abound in processed food and have been implicated in the pathology of diverse diseases including gut, cardiovascular, and cancer diseases. Recently, RNA Sequencing (RNA-seq) has been widely used to profile gene expression. To characterize gene expression and
E Wieland et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(13), 5929-5933 (1993-07-01)
Oxidative modification of low density lipoprotein is believed to be an important pathway by which the lipoprotein becomes atherogenic. The in vitro systems for oxidative modification of low density lipoprotein thus far described all appear to depend upon the presence