L9655

Linoleic Acid-Oleic Acid-Albumin, 100x

Name: Linoleic Acid-Oleic Acid-Albumin, 100x
Brand: SIGMA

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Fatty Acid-Albumin-complexes

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352211

Product Name

Linoleic Acid-Oleic Acid-Albumin, 100x, liquid, sterile-filtered, BioReagent, suitable for cell culture

biological source

protein from bovine

sterility

sterile-filtered

product line

BioReagent

form

liquid

composition

linoleic acid and oleic acid, 2 mol/mol albumin (each)

concentration

100 mg/mL BSA in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

Quality Level

300

Application

Linoleic Acid-Oleic Acid-Albumin, 100x has been used:

to induce acute hepatocellular stress in primary hepatocytes
to induce oxidative stress in cytotrophoblasts
to induce lipid droplet accumulation in HepG2 cells

General description

Fatty Acid-Albumin-complexes have been employed as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Such proteins may release beneficial fatty acids and bind those that are inhibitory. Oleic acid bound to bovine serum albumin (BSA) is beneficial to the growth of a variety of cell types (e.g., baby hamster kidney (BHK) hybridoma). A mixture of poly-unsaturated and monosaturated fatty acids (i.e., linoleic acid and oleic acid) produces synergistic effect when used as a media supplement.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Hepatocyte-specific Keap1 deletion reduces liver steatosis but not inflammation during non-alcoholic steatohepatitis development.

Pierluigi Ramadori et al.

Free radical biology & medicine, 91, 114-126 (2015-12-25)

Generation of reactive oxygen species (ROS) in response to fatty acids accumulation has been classically proposed as a possible "second hit" triggering progression from simple steatosis to non-alcoholic steatohepatitis (NASH). In this study we challenged hepatocyte-specific Keap1 knockout mice (Keap1(Δhepa))

Epigenetic regulation of carnitine palmitoyltransferase 1 (Cpt1a) by high fat diet.

Laura Moody et al.

Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(2), 141-152 (2019-01-04)

Carnitine palmitoyltransferase 1 (Cpt1a) is a rate-limiting enzyme that mediates the transport of fatty acids into the mitochondria for subsequent beta-oxidation. The objective of this study was to uncover how diet mediates the transcriptional regulation of Cpt1a. Pregnant Sprague Dawley

Molecular Characterization of Microtubule Affinity-Regulating Kinase4 from Sus scrofa and Promotion of Lipogenesis in Primary Porcine Placental Trophoblasts.

Liang Tian et al.

International journal of molecular sciences, 20(5) (2019-03-13)

This study aimed to characterize the full-length cDNA of MARK4 in Sus scrofa, and evaluated its potential role in the regulation of lipid accumulation in pig placental trophoblasts and analyzed signaling pathways involved, thereby providing insights into mechanisms for placental

GDF11 rapidly increases lipid accumulation in liver cancer cells through ALK5-dependent signaling.

Jan Frohlich et al.

Biochimica et biophysica acta. Molecular and cell biology of lipids, 1866(6), 158920-158920 (2021-03-09)

Hepatocellular carcinoma (HCC) is one of the fastest-growing causes of cancer-related mortalities worldwide and this trend is mimicked by the surge of non-alcoholic fatty liver disease (NAFLD). Altered hepatic lipid metabolism promotes HCC development through inflammation and activation of oncogenes.

A developed serum-free medium and an optimized chemical cocktail for direct conversion of human dermal fibroblasts into brown adipocytes.

Yukimasa Takeda et al.

Scientific reports, 10(1), 3775-3775 (2020-03-01)

Brown adipocytes coordinate systemic energy metabolism associated with the pathogenesis of obesity and related metabolic diseases including type 2 diabetes. We have previously reported chemical compound-induced brown adipocytes (ciBAs) converted from human dermal fibroblasts without using transgenes. In this study

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Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Oleic Acid in Cell Culture

How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.