M0643

Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

Product Name

Minimum Essential Medium Eagle, With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
Earle’s salts (5% CO₂): yes
NaHCO₃: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

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Related Categories

Cell Culture & Analysis

Cell Culture Media & Buffers

Classical Media & Buffers

Minimum Essential Medium (MEM)

Molecular Biology & Functional Genomics

Application

Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63, dispersed ventricular cells, and endometrial adenocarcinoma Ishikawa cells.

General description

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids. It is widely used as a media formulation for many cell culture studies. It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000500472

0000500470

25D291

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Effects of terpenoid phenol derivatives on calcium current in canine and human ventricular cardiomyocytes.

János Magyar et al.

European journal of pharmacology, 487(1-3), 29-36 (2004-03-23)

Concentration-dependent (10-1000 microM) effects of terpenoid phenol derivatives were studied on L-type Ca(2+) current in isolated canine and human ventricular cardiomyocytes using the whole-cell configuration of patch clamp technique. Carvacrol, thymol and eugenol suppressed peak Ca(2+) current at +5 mV

Animal-cell culture media: History, characteristics, and current issues.

Tatsuma Yao et al.

Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)

Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google

Is it time to reinvent basic cell culture medium?

Turney J McKee et al.

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