Skip to Content
Merck

M1255

Calcein Blue

Powder

Synonym(s):

4-Methylumbelliferone-8-methyliminodiacetic acid

Sign In to View Organizational & Contract Pricing.

Select a Size

All Photos(3)

About This Item

Empirical Formula (Hill Notation):
C15H15NO7
CAS Number:
54375-47-2
Molecular Weight:
321.28
NACRES:
NA.47
PubChem Substance ID:
24896629
UNSPSC Code:
12171500
EC Number:
259-129-8
MDL number:
MFCD00006867

Product Name

Calcein Blue, fluorescent dye

InChI key

RJPSHDMGSVVHFA-UHFFFAOYSA-N

InChI

1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)

SMILES string

CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12

form

powder

technique(s)

titration: suitable

color

white to light yellow

solubility

1 M NaOH: 50 mg/mL

fluorescence

λex 322 nm; λem 445 nm in methanol

suitability

suitable for indicator for EDTA titration

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.

General description

Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000444922
0000444922
0000377014
0000353433
0000326239

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

T Lymphocytes Influence the Mineralization Process of Bone.
El Khassawna T
Frontiers in Immunology, 8, 562-562 (2017)
Indicators: International Series of Monographs in Analytical Chemistry (2013)
Zachary J Reitman et al.
Nature communications, 10(1), 3731-3731 (2019-08-21)
Pilocytic astrocytoma (PA), the most common childhood brain tumor, is a low-grade glioma with a single driver BRAF rearrangement. Here, we perform scRNAseq in six PAs using methods that enabled detection of the rearrangement. When compared to higher-grade gliomas, a
Tobias A Fuchs et al.
The Journal of cell biology, 176(2), 231-241 (2007-01-11)
Neutrophil extracellular traps (NETs) are extracellular structures composed of chromatin and granule proteins that bind and kill microorganisms. We show that upon stimulation, the nuclei of neutrophils lose their shape, and the eu- and heterochromatin homogenize. Later, the nuclear envelope
Xiaomian Wu et al.
International journal of nanomedicine, 7, 1215-1225 (2012-03-16)
Compared with titanium (Ti) and other metal implant materials, poly(ether-ether ketone) (PEEK) shows outstanding biomechanical properties. A number of studies have also reported attractive bioactivity for nano-TiO(2) (n-TiO(2)). In this study, n-TiO(2)/PEEK nanocomposites were prepared, taking advantage of the unique
View All Related Papers

Articles

Sensor Dyes

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service