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Merck

M1533

N,N′-Methylenebisacrylamide solution

suitable for electrophoresis, 2% in H2O

Synonym(s):

BIS

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About This Item

Linear Formula:
(CH2=CHCONH)2CH2
CAS Number:
110-26-9
Molecular Weight:
154.17
UNSPSC Code:
41105319
NACRES:
NB.22
PubChem Substance ID:
24896663
MDL number:
MFCD00008625
Beilstein/REAXYS Number:
1706297

Product Name

N,N′-Methylenebisacrylamide solution, suitable for electrophoresis, 2% in H2O

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

SMILES string

C=CC(=O)NCNC(=O)C=C

InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

sterility

0.2 μm filtered

form

liquid

concentration

2% in H2O

technique(s)

electrophoresis: suitable

storage temp.

2-8°C

Quality Level

200

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Application

N,N′-Methylenebisacrylamide solution has been used as a component of hydrogel precursor solution and magnified analysis of the proteome (MAP) gel solution during hydrogel fabrication. It has also been used as a component of re-embedding solution during re-embedding of expanded hydrogels.
To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

General description

N,N′-Methylenebisacrylamide solution is a cross-linking agent used in polyacrylamide gel preparation.

Preparation Note

Prepared from electrophoresis grade bis-acrylamide in ultra-pure water.

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Carc. 1B - Muta. 1B - STOT RE 2 Oral

target_organs

Peripheral nervous system

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000512038
0000512038
0000450166
0000450163
0000450166

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Chapter 15 - Techniques for Protein Analysis
Barh D and Azevedo V
Omics : A Journal of Integrative Biology null
Jean Guglielminotti et al.
Anesthesiology, 122(5), 985-993 (2015-03-03)
Individual assessment of the amplitude of a physiologic reflex evoked by a standardized noxious test (SNT) before surgical stimulation has been suggested to predict movement upon the forthcoming surgical stimulation. This study aimed to compare the ability of pupillary dilatation
A J Aho et al.
British journal of anaesthesia, 115(2), 258-266 (2015-07-04)
Concomitantly recorded Bispectral Index® (BIS) and Entropy™ values sometimes show discordant trends during general anaesthesia. Previously, no attempt had been made to discover which EEG characteristics cause discrepancies between BIS and Entropy. We compared BIS and Entropy values, and analysed
Alice E Stanton et al.
Acta biomaterialia, 96, 310-320 (2019-07-01)
Extracellular matrix (ECM) is comprised of different types of proteins, which change in composition and ratios during morphogenesis and disease progression. ECM proteins provide cell adhesion and impart mechanical cues to the cells. Increasing substrate stiffness has been shown to
Bjørn Bjorvatn et al.
Sleep & breathing = Schlaf & Atmung, 19(4), 1387-1393 (2015-04-10)
The objective of the present study was to investigate the prevalence of insomnia and excessive sleepiness in relation to the presence and severity of obstructive sleep apnea (OSA). The sample consisted of patients referred to a university hospital on suspicion
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