M3769

Medium 199

Name: Medium 199
Brand: SIGMA

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

Synonym(s):

M199 Medium, TCM 199

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About This Item

NACRES:

NA.71

UNSPSC Code:

12352207

Product Name

Medium 199, Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO₂): yes
NaHCO₃: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

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Related Categories

Biochemicals

Biological Buffers

Cell Culture & Analysis

Cell Culture Media & Buffers

Classical Media & Buffers

Application

Medium 199 has been used:

to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Preparation Note

Formulated to contain 9.4 grams of powder per liter of medium.

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000437175

0000436546

0000388915

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Parameters Affecting Continuous In Vitro Culture of Treponema pallidum Strains.

Diane G Edmondson et al.

mBio, 12(1) (2021-02-25)

The bacterium that causes syphilis, Treponema pallidum subsp. pallidum, has now been cultured in vitro continuously for periods exceeding 3 years using a system consisting of coculture with Sf1Ep rabbit epithelial cells in TpCM-2 medium and a low-oxygen environment. In

New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs.

Luciana de Lima Ferreira et al.

PLoS neglected tropical diseases, 14(12), e0009015-e0009015 (2020-12-29)

Trypanosoma rangeli is a non-pathogenic protozoan parasite that infects mammals, including humans, in Chagas disease-endemic areas of South and Central America. The parasite is transmitted to a mammalian host when an infected triatomine injects metacyclic trypomastigotes into the host's skin

Organosilica colloids as nitric oxide carriers: Pharmacokinetics and biocompatibility.

Hung-Chang Chou et al.

Colloids and surfaces. B, Biointerfaces, 208, 112136-112136 (2021-10-11)

Nitric oxide (NO) is a potential therapeutic agent for various diseases. However, it is challenging to deliver this unstable, free-radical gaseous molecule in the body. Various nanoparticle-based drug delivery systems have been investigated as promising NO carriers without detailed characterization

Alanyl-glutamine dipeptide restores the cytoprotective stress proteome of mesothelial cells exposed to peritoneal dialysis fluids.

Klaus Kratochwill et al.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 27(3), 937-946 (2011-08-23)

Exposure of mesothelial cells to peritoneal dialysis fluids (PDF) results in cytoprotective cellular stress responses (CSR) that counteract PDF-induced damage. In this study, we tested the hypothesis that the CSR may be inadequate in relevant models of peritoneal dialysis (PD)

Newly defined conditions for the in vitro cultivation and cryopreservation of Dientamoeba fragilis: new techniques set to fast track molecular studies on this organism.

J L N Barratt et al.

Parasitology, 137(13), 1867-1878 (2010-07-09)

Dientamoeba fragilis is a pathogen of the human gastrointestinal tract that is a common cause of diarrhoea. A paucity of knowledge on the in vitro cultivation and cryopreservation of Dientamoeba has meant that few studies have been conducted to investigate

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Articles

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.