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Merck

N7653

p-Nitrophenyl Phosphate Liquid Substrate System

alkaline phosphatase substrate, liquid

Synonym(s):

4-Nitrophenyl phosphate disodium salt solution, pNPP

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About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2
CAS Number:
4264-83-9
Molecular Weight:
263.05
UNSPSC Code:
12352204
PubChem Substance ID:
24897835
NACRES:
NA.83
MDL number:
MFCD00007319

Key Documents

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Product Name

p-Nitrophenyl Phosphate Liquid Substrate System, liquid

SMILES string

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI key

VIYFPAMJCJLZKD-UHFFFAOYSA-L

form

liquid

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

Related Categories

General description

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Physical form

Ready-to-use.

Disclaimer

Stable at least one year when stored below 0 °C.

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

target_organs

Kidney,Liver,Blood

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

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Certificates of Analysis (COA)

Lot/Batch Number
SLCN6783
SLCN6783
SLCL1721
SLCF2308
SLBP4121V

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J V Jones et al.
Journal of immunological methods, 118(1), 79-84 (1989-03-10)
We have recently described a substrate amplification system, based on the method of Self, which increases the sensitivity of alkaline phosphatase (AP)-dependent enzyme-linked immunosorbent assays (ELISA) by a factor of 30-50. This increase is achieved by having AP, the primary
Evaluation of selective laser sintered polyamide/hydroxyapatite composite compositions-in vitro and in vivo
Kamarajan, et al.
International journal of biomedical research, 80(8), 467-474 (2007)
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Boado RJ, et al.
Biotechnology and Bioengineering, 96(2), 381-391 (2007)
I K Manoylov et al.
Clinical and experimental immunology, 197(3), 329-340 (2019-04-23)
Type 1 diabetes mellitus is an autoimmune metabolic disorder characterized by chronic hyperglycemia, the presence of autoreactive T and B cells and autoantibodies against self-antigens. A membrane-bound enzyme on the pancreatic beta-cells, glutamic acid decarboxylase 65 (GAD65), is one of
Peter Van den Eede et al.
PloS one, 6(1), e16257-e16257 (2011-02-08)
There is an increasing body of literature reporting treatment failure of the currently recommended radical treatment of Plasmodium vivax infections. As P. vivax is the main malaria species outside the African continent, emerging tolerance to its radical treatment regime could
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