P0609

Pepsin−Agarose from porcine gastric mucosa

Name: Pepsin−Agarose from porcine gastric mucosa
Brand: SIGMA

lyophilized powder, ≥30 units/mg dry solid

Synonym(s):

Pepsin A

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All Photos(3)

About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.23.1 (BRENDA, IUBMB)

MDL number:

MFCD00081840

Product Name

Pepsin−Agarose from porcine gastric mucosa, lyophilized powder, ≥30 units/mg dry solid

biological source

Porcine gastric mucosa

form

lyophilized powder

specific activity

≥30 units/mg dry solid

mol wt

35 kDa

matrix

4% cross-linked beaded agarose

UniProt accession no.

P00791

storage temp.

−20°C

Application

Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin.

Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG.

Used to produce F(ab′)₂ fragments of antibodies.¹

Biochem/physiol Actions

Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.

General description

Pepsin is an enzyme found in gastric secretion.

Other Notes

One unit will produce a ΔA₂₈₀ of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)

Physical form

Lyophilized powder stabilized with lactose

Preparation Note

One gram solid swells to 5-10ml packed gel.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P304 + P340 - P342 + P311 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Activation of macrophages by gliadin fragments: isolation and characterization of active peptide.

Ludmila Tucková et al.

Journal of leukocyte biology, 71(4), 625-631 (2002-04-03)

Celiac disease, induced by dietary gluten, is characterized by mucosal atrophy and local inflammation associated with cell infiltration and activation. Unlike other food proteins, gluten and its proteolytic fragments, besides inducing a specific immune response, were shown to activate components

Induction of mucosal tolerance in Peyer's patch-deficient, ligated small bowel loops.

Thomas A Kraus et al.

The Journal of clinical investigation, 115(8), 2234-2243 (2005-07-26)

To explore the requirement for M cells and the Peyer's patch (PP) in induction of oral tolerance and address the potential in vivo role of intestinal epithelial cells as nonprofessional APCs, we have attempted to induce tolerance in mice with

Effects of aging and gastritis on gastric acid and pepsin secretion in humans: a prospective study.

M Feldman et al.

Gastroenterology, 110(4), 1043-1052 (1996-04-01)

Recent studies suggesting that gastric secretion does not decrease with aging included few elderly individuals and measured only acid secretion. The aims of this study were to measure gastric acid and pepsin output in 206 health Americans (age range, 18-98

Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides.

Widya A Wahab et al.

International journal of experimental pathology, 97(4), 303-309 (2016-09-24)

Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin

Isoforms of Cathepsin B1 in Neurotropic Schistosomula of Trichobilharzia regenti Differ in Substrate Preferences and a Highly Expressed Catalytically Inactive Paralog Binds Cystatin.

Hana Dvořáková et al.

Frontiers in cellular and infection microbiology, 10, 66-66 (2020-03-17)

Schistosomula (the post-infective stages) of the neurotropic schistosome Trichobilharzia regenti possess multiple isoforms of cathepsin B1 peptidase (TrCB1.1-TrCB1.6) with involvement in nutrient digestion. The comparison of substrate preferences of TrCB1.1 and TrCB1.4 showed that TrCB1.4 had a very narrow substrate

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Articles

Antibody Fragmentation with Pepsin Digestion

Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).

Protocols

Enzymatic Assay of Pepsin (3.4.23.1)

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.