P2860

Monoclonal Anti-PSF antibody produced in mouse

Name: Monoclonal Anti-PSF antibody produced in mouse
Brand: SIGMA

clone B92, purified from hybridoma cell culture

Synonym(s):

Mouse Anti-Polypyrimidine tract-binding protein-associated splicing factor

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03457573

Product Name

Monoclonal Anti-PSF antibody produced in mouse, clone B92, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B92, monoclonal

form

buffered aqueous solution

species reactivity

mouse, human

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
radioimmunoassay: suitable using cells
western blot: 2-4 μg/mL using whole extract of cultured HeLa cells

isotype

IgG1

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TAP1(6890)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)

Monoclonal Anti-PSF antibody produced in mouse has been used in:

immunofluorescence
immunohistochemistry
immunoprecipitation

Biochem/physiol Actions

Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues.

Immunogen

lysate of mouse bone marrow-derived stromal cell line.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000445884

0000410510

0000243307

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Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice

Nakagawa S, et al.

The Journal of cell biology, 193(1), 31-39 (2011)

Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB

Meissner M, et al.

Journal of Cellular Biochemistry, 76(4), 559-566 (2000)

Cytoplasmic Relocalization of TAR DNA-Binding Protein 43 Is Not Sufficient to Reproduce Cellular Pathologies Associated with ALS In vitro

Wobst HJ, et al.

Frontiers in Molecular Neuroscience, 10(7) (2017)

Acute hepatotoxicity of 2' fluoro-modified 5-10-5 gapmer phosphorothioate oligonucleotides in mice correlates with intracellular protein binding and the loss of DBHS proteins.

Wen Shen et al.

Nucleic acids research, 46(5), 2204-2217 (2018-02-02)

We reported previously that a 2' fluoro-modified (2' F) phosphorothioate (PS) antisense oligonucleotides (ASOs) with 5-10-5 gapmer configuration interacted with proteins from Drosophila behavior/human splicing (DBHS) family with higher affinity than PS-ASOs modified with 2'-O-(2-methoxyethyl) (2' MOE) or 2',4'-constrained 2'-O-ethyl

Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells.

Feng Chi et al.

PloS one, 7(4), e35862-e35862 (2012-04-27)

IbeA-induced NF-κB signaling through its primary receptor vimentin as well as its co-receptor PSF is required for meningitic E. coli K1 penetration and leukocyte transmigration across the blood-brain barrier (BBB), which are the hallmarks of bacterial meningitis. However, it is

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