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Merck

P3910

Protease from Bacillus licheniformis

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

Synonym(s):

Proteinase from Bacillus licheniformis, Subtilisin A

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About This Item

CAS Number:
9014-01-1
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-752-2
NACRES:
NA.84
MDL number:
MFCD00132092
EC Number:
3.4.21.62 (BRENDA, IUBMB)

Product Name

Protease from Bacillus licheniformis, lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

form

lyophilized powder

quality

for use in Total Dietary Fiber Assay, TDF-100A

mol wt

27 kDa

application(s)

food and beverages

shipped in

wet ice

storage temp.

2-8°C

Quality Level

100

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Application

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

Biochem/physiol Actions

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

General description

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Other Notes

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per minute at pH 7.5 at 37°C.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000519687
0000519687
0000519281
0000519281
0000505224

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Katja Kantanen et al.
Foods (Basel, Switzerland), 11(9) (2022-05-15)
Faba bean is a potential ingredient due to its high protein yield and its possible cultivation in colder climate regions. In this study, meat analogues made from faba bean protein isolate (FPI) and concentrate (FPC) blends were produced using high
John Szpylka et al.
Journal of AOAC International, 95(6), 1547-1554 (2013-03-05)
The method for "Determination of Total Folates in Infant Formula and Adult Nutritionals by Trienzyme Extraction and UPLC-MS/MS Quantitation" was submitted to the Folate Working Group for consideration for adoption as Official First Action by AOAC INTERNATIONAL. This method uses
Zhaoyue Men et al.
Journal of animal science and biotechnology, 13(1), 14-14 (2022-01-17)
Dietary fiber (DF) is often eschewed in swine diet due to its anti-nutritional effects, but DF is attracting growing attention for its reproductive benefits. The objective of this study was to investigate the effects of DF intake level on oocyte
Micaela Vannini et al.
Polymers, 13(7) (2021-04-04)
With the aim to fully exploit the by-products obtained after the industrial extraction of starch from sweet potatoes, a cascading approach was developed to extract high-value molecules, such as proteins and pectins, and to valorize the solid fraction, rich in
Lisete Paiva et al.
Marine drugs, 16(8) (2018-07-28)
This study evaluates, for the first time, the seasonal (winter and summer) and geographical (São Miguel⁻SMG and Santa Maria⁻SMA Islands) variability of Fucus spiralis (Fs) biochemical composition (dry weight basis) and antioxidant properties. Protein and carbohydrates presented higher values in
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