P4069

Phosphatase, Alkaline from Escherichia coli

Name: Phosphatase, Alkaline from <I>Escherichia coli</I>
Brand: SIGMA

buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

Synonym(s):

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

Select a Size

All Photos(1)

About This Item

CAS Number:

9001-78-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-631-4

MDL number:

MFCD00131849

EC Number:

3.1.3.1 (BRENDA, IUBMB)

Product Name

Phosphatase, Alkaline from Escherichia coli, buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

biological source

Escherichia coli

form

buffered aqueous glycerol solution

specific activity

20-50 units/mg protein (in glycine buffer)

foreign activity

DNase, RNase, none detected

storage temp.

−20°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Proteins & Enzymes

Detection Substrates & Enzymes

Analysis Note

Protein determined by biuret.

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution.

Phosphatase, Alkaline from Escherichia coli has been used:

in fluorometric assay to determine its enzyme activity
in the dephosphorylating enzyme cocktail for dephosphorylation of peptides
in treating the nerve and HL60 cell lysate for β-hydroxy β-methylglutaryl-Coenzyme A (HMG-CoA) reductase activity

Biochem/physiol Actions

Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Disclaimer

Alkaline phosphatase in 5 mM EDTA, pH 8, will be irreversibly heat inactivated at 75°C for 10 minutes.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Physical form

Solution in 5 mM Tris-HCl, pH 7.4, 0.5 mM MgCl₂, 0.5 mM ZnCl₂, 50% (w/v) glycerol

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The prion protein and cellular cholesterol homeostasis

Diomede L, et al.

Neurobiology of Lipids, 1(2), 8-14 (2002)

Sensitive fluorogenic substrate for alkaline phosphatase

Levine MN and Raines RT

Analytical Biochemistry, 418(2), 247-252 (2011)

Phospho-iTRAQ data article: Assessing isobaric labels for the large-scale study of phosphopeptide stoichiometry

Glibert P, et al.

Data in Brief, 4(2), 60-65 (2015)

Enrichment of C-terminal fragments in TAR DNA-binding protein-43 cytoplasmic inclusions in brain but not in spinal cord of frontotemporal lobar degeneration and amyotrophic lateral sclerosis.

Lionel M Igaz et al.

The American journal of pathology, 173(1), 182-194 (2008-06-07)

TAR DNA-binding protein (TDP-43) has been recently described as a major pathological protein in both frontotemporal dementia with ubiquitin-positive inclusions (FTLD-U) and amyotrophic lateral sclerosis. However, little is known about the relative abundance and distribution of different pathological TDP-43 species

IL-6 alters osteocyte signaling toward osteoblasts but not osteoclasts.

A D Bakker et al.

Journal of dental research, 93(4), 394-399 (2014-02-05)

Mechanosensitive osteocytes regulate bone mass in adults. Interleukin 6 (IL-6), such as present during orthodontic tooth movement, also strongly affects bone mass, but little is known about the effect of IL-6 on osteocyte function. Therefore we aimed to determine in

View All Related Papers