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P4252

Phosphatase, Alkaline from Escherichia coli

ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

Synonym(s):

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS Number:
9001-78-9
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
MFCD00131849
EC Number:
3.1.3.1 (BRENDA, IUBMB)

Product Name

Phosphatase, Alkaline from Escherichia coli, ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

biological source

Escherichia coli

form

ammonium sulfate suspension

specific activity

30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

storage temp.

2-8°C

Quality Level

300

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Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 is an ammonium sulfate suspension in glycine buffer.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA . Product P4252 is provided in the ratio of 30-90 units/mg of protein.

Analysis Note

The activity at pH 8.0 at 25 °C is approximately the same as at pH 10.4 at 37 °C.

Biochem/physiol Actions

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Disclaimer

Presence of 0.1% SDS in the alkaline phosphatase assays will inhibit any nuclease contamination in the sample.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Physical form

Suspension in 2.5 M (NH4)2SO4

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000486032
0000486032
0000422145
0000422145
0000121032

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E. coli Alkaline Phosphatase
1.Reid, T., and Wilson
The Enzymes, 4, 373-373 (1971)
Tamaha Yamaguchi et al.
Frontiers in microbiology, 11, 570081-570081 (2021-02-09)
The labile fraction of dissolved organic phosphorus (DOP) - predominantly consisting of phosphoric esters - is an important microbial P source in the subtropical oligotrophic ocean. However, unlike phosphate, knowledge for labile DOP is still limited due to the scarcity
The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA
Basma El Yacoubi, Benjamin Lyons, et al.
RNA, 37, 2894-2909 (2009)
Alkaline phosphatase from Thermotoga neapolitana.
A Savchenko et al.
Methods in enzymology, 331, 298-305 (2001-03-27)
Wenqi Xu et al.
Nature, 591(7849), 317-321 (2021-01-29)
METTL3 (methyltransferase-like 3) mediates the N6-methyladenosine (m6A) methylation of mRNA, which affects the stability of mRNA and its translation into protein1. METTL3 also binds chromatin2-4, but the role of METTL3 and m6A methylation in chromatin is not fully understood. Here
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