P6181

Glu-C Protease

Name: Glu-C Protease
Brand: SIGMA

Cleaves at glutamic acid residues, suitable for Mass Spectrometry, from Staphylococcus aureus V8

Synonym(s):

V8 Protease

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All Photos(1)

About This Item

CAS Number:

66676-43-5

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

200-350-6

MDL number:

MFCD01324998

EC Number:

3.4.21.19 (BRENDA, IUBMB)

Product Name

Endoproteinase Glu-C from Staphylococcus aureus V8, suitable for protein sequencing, lyophilized powder

grade

Proteomics Grade

form

lyophilized powder

analyte chemical class(es)

amino acids

suitability

suitable for protein sequencing

storage temp.

−20°C

Quality Level

200

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Related Categories

Protein Mass Spectrometry

Application

Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for:

obtaining proteolytic cleavage fragments of the S-layer protein (from Bacillus stearothermophilus ATCC 12980) to perform affinity studies.
the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination.
limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase).
the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.

Biochem/physiol Actions

Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine endoprotease, which hydrolyzes peptide bonds at the carboxyl side of glutamyl and aspartyl residues. The specificity of Glu-C is dependent upon the buffer and pH employed as well as the structure around the potential cleavage site. In ammonium acetate (pH 4.0) or ammonium bicarbonate (pH 7.8), the enzyme preferentially cleaves glutamyl bonds; whereas, in phosphate buffer (pH 7.8) Glu-C will cleave at either site. Glu-C is reported to be active in the presence of 0.2% SDS (sodium dodecyl sulfate) and in 4.0M urea.

pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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The Protein Protocols Handbook (1996)

Isolation of a Protein Containing Covalently Linked Large and Small Subunits of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from Botryococcus braunii.

X. Wang et al.

Plant physiology, 111(2), 441-445 (1996-06-01)

Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and a 66-kD protein were co-purified from solubilized microsomal preparations of the green alga Botryococcus braunii by Green A agarose, sucrose density gradient, MonoQ, and gel filtration. The 66-kD protein remained intact after 6 M urea treatment

The abalone egg vitelline envelope receptor for sperm lysin is a giant multivalent molecule.

Swanson, W.J., and Vacquier, V.D.

Proceedings of the National Academy of Sciences of the USA, 94(13), 6724-6729 (1999)

S-layer gene sbsC of Bacillus stearothermophilus ATCC 12980: molecular characterization and heterologous expression in Escherichia coli.

M Jarosch et al.

Microbiology (Reading, England), 146 ( Pt 2), 273-281 (2000-03-09)

The cell surface of Bacillus stearothermophilus ATCC 12980 is completely covered with an oblique S-layer lattice. To investigate sequence identities and a common structure-function relationship in S-layer proteins of different B. stearothermophilus wild-type strains, the nucleotide sequence encoding the S-layer

Kenneth AW

Human Genome Methods (1997)

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