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Merck

P7181

Polyethylene glycol solution

Hybri-Max™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

Synonym(s):

PEG Solution, Polyethylene Glycol

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About This Item

MDL number:
MFCD00081839
UNSPSC Code:
12161902
PubChem Substance ID:
24898829
NACRES:
NA.75

Product Name

Polyethylene glycol solution, Hybri-Max™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

SMILES string

C(CO)O

grade

Hybri-Max™

sterility

0.2 μm filtered

product line

BioReagent

form

solution

mol wt

average mol wt 1,450

concentration

50 % (w/v)

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

Quality Level

200

Related Categories

Application

Polyethylene glycol solution has been used in the hybridization of the following:
  • irradiated camel fibroblast cells with A23 hamster cells
  • mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
  • rabbit splenocytes and cell line 240E-1

Recommended for use in a normal fusion protocol requiring 50% PEG.

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

Biochem/physiol Actions

Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.

General description

Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.

Other Notes

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.

Packaging

Packaged in sealed ampules under nitrogen.

Preparation Note

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

212.0 °F - closed cup

flash_point_c

100 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
RNBK9585
RNBK0589
RNBH8345
RNBG7124
RNBG0388

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Acoustic, thermal and molecular interactions of polyethylene glycol (2000, 3000, 6000)
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