R6626

Rhodamine B

Name: Rhodamine B
Brand: SIGMA

≥95% purity (HPLC), powder

Synonym(s):

Basic Violet 10, Brilliant Pink B, Rhodamine O, Tetraethylrhodamine

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All Photos(4)

About This Item

Empirical Formula (Hill Notation):

C₂₈H₃₁ClN₂O₃

CAS Number:

81-88-9

Molecular Weight:

479.01

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24899386

EC Number:

201-383-9

MDL number:

MFCD00011931

Colour Index Number:

45170

Beilstein/REAXYS Number:

4091619

Product Name

Rhodamine B, ≥95% (HPLC)

InChI key

PYWVYCXTNDRMGF-UHFFFAOYSA-N

InChI

1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccccc4C(O)=O)=[N+](/CC)CC)c1

assay

≥95% (HPLC)

form

powder

technique(s)

titration: suitable

color

faint green to very dark green

mp

210-211 (dec.) (lit.)

solubility

H₂O: 1 mg/mL

density

0.79 g/cm³

ε (extinction coefficient)

104500-115800 at 542-554 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Clinical & Diagnostics

Hematology Stains

Histology Stains

Tissue Diagnostics

Application

Rhodamine B has been used:

to represent fluorescence of tetra methyl rhodamine (TMR)
in the preparation of adhesive resin for confocal laser scanning microscopy
as a fluorescent dye in intrahemolymph dye dosing and retinal image acquisition
to depict brain-specific dye capture
for imaging targets

General description

Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a colorant in textiles and food stuffs. Rhodamine B is associated with eye, skin and respiratory tract irritation. It is used as a biomarker in oral rabies vaccine.

pictograms

GHS05,GHS07

signalword

Danger

hcodes

H302,H318,H412

pcodes

P264 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P501

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

0000522379

0000509191

0000505986

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Novel models for studying the blood-brain and blood-eye barriers in Drosophila

Nag S, et al.

The Blood-Brain and Other Neural Barriers, 357-369 (2011)

Sulfadimethoxine and rhodamine B as oral biomarkers for European badgers (Meles meles)

Southey AK, et al.

Journal of Wildlife Diseases, 38(2), 378-384 (2002)

John F. Breidenstein

A study of water circulation in Monterey harbor using rhodamine B dye (1965)

Palmitate mediated diacylglycerol accumulation causes endoplasmic reticulum stress, Plin2 degradation, and cell death in H9C2 cardiomyoblasts

AliAkoumi

Experimental Cell Research (2017)

5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells

HanGoo Kang

Nano-Micro Letters (2016)

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