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Merck

S1402

Sulforhodamine B sodium salt

powder, BioReagent, suitable for cell culture

Synonym(s):

Kiton Red 620

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About This Item

Empirical Formula (Hill Notation):
C27H29N2NaO7S2
CAS Number:
3520-42-1
Molecular Weight:
580.65
EC Number:
222-529-8
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
24899477
MDL number:
MFCD00010180
Colour Index Number:
45100
Beilstein/REAXYS Number:
3886008

Product Name

Sulforhodamine B sodium salt, powder, BioReagent, suitable for cell culture

InChI key

SXQCTESRRZBPHJ-UHFFFAOYSA-M

InChI

1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1

SMILES string

[Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1

biological source

synthetic (organic)

product line

BioReagent

form

powder

composition

Dye content, ~75%

technique(s)

cell culture | mammalian: suitable

solubility

methanol: 1 mg/mL

fluorescence

λex 565 nm; λem 586 nm in H2O

Quality Level

100

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Application

Sulforhodamine B sodium salt has been used:
  • for relative total cell mass quantification
  • to investigate the viscosity of coacervates
  • as a molecular rotor for microviscosity determination in aerosol droplets

Biochem/physiol Actions

Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening.

General description

Sulforhodamine B is a rhodamine derived aminoxanthene dye. It contains two sulfonic acids groups which separate in alkaline condition.

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT3818
SHBR3296
SHBN2223
SHBL9542
MKCD3873

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Bioconjugate Techniques, 317-317 (1996)
Issues in Pharmacology, Pharmacy, Drug Research, and Drug Innovation (2012)
Luca Giordano et al.
American journal of respiratory cell and molecular biology, 60(5), 515-522 (2018-10-20)
Cigarette smoke (CS) exposure is the predominant risk factor for the development of chronic obstructive pulmonary disease (COPD) and the third leading cause of death worldwide. We aimed to elucidate whether mitochondrial respiratory inhibition and oxidative stress are triggers in
Fluorescent lifetime imaging of atmospheric aerosols: a direct probe of aerosol viscosity
Faraday Discussions, 165, 343-356 (2013)
Quantitative sensing of microviscosity in protocells and amyloid materials using fluorescence lifetime imaging of molecular rotors
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 8947, 89471C-89471C (2014)
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