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Merck

S5394

Lipoprotein Deficient Serum from fetal calf

frozen liquid

Synonym(s):

Fetal Calf Serum, Lipoprotein Deficient Serum

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About This Item

NACRES:
NA.61
UNSPSC Code:
12352207
biological source:
bovine fetus
form:
frozen liquid
shipped in:
dry ice

Product Name

Lipoprotein Deficient Serum from fetal calf, frozen liquid

biological source

bovine fetus

form

frozen liquid

concentration

≥14 mg protein/mL Biuret

Quality Level

200

impurities

<1 ng/mg protein endotoxin
<5 % of normal lipoprotein

shipped in

dry ice

storage temp.

−20°C

Related Categories

Application

Lipoprotein Deficient Serum from fetal calf has been used as a supplement in culture media to grow Chinese hamster ovary (CHO)-derived cells, thymic lobes and human peripheral blood mononuclear cells (PBMCs).

General description

Studies show that the production of sterols is reduced in human fibroblasts cultured in the presence of lipoprotein deficient serum.

Preparation Note

Dialyzed against Dulbecco′s phosphate buffered saline, pH 7.4-7.5
0.2 μm filtered

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ5148
SLBW0309
SLBQ5608V
SLBN4785V
SLBL5464V

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Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP)
Shao W and Espenshade PJ
The Journal of Biological Chemistry, 289(11), 7547-7557 (2014)
Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage
Komaniwa S, et al.
European Journal of Immunology, 39(1), 96-112 (2009)
P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Brian T Emmer et al.
PLoS genetics, 17(1), e1009285-e1009285 (2021-01-30)
Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
Liliana M R Silva et al.
Frontiers in cellular and infection microbiology, 12, 809606-809606 (2022-03-01)
Apicomplexan parasites are considered as defective in cholesterol synthesis. Consequently, they need to scavenge cholesterol from the host cell by either enhancing the uptake of extracellular cholesterol sources or by upregulating host cellular de-novo biosynthesis. Given that Eimeria bovis macromeront
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Protocols

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

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