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Merck

S8884

Safranin O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A

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About This Item

Empirical Formula (Hill Notation):
C20H19ClN4
CAS Number:
477-73-6
Molecular Weight:
350.84
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24899820
EC Number:
207-518-8
MDL number:
MFCD00011759
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099

Product Name

Safranin O, certified by the BSC

InChI key

OARRHUQTFTUEOS-UHFFFAOYSA-N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

SMILES string

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

agency

certified by the BSC

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

green to very dark green

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Application

Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.
Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.
Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.

General description

Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT2172
0000505979
0000505978
0000505978
0000505979

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Isolation of Multipotent Mesenchymal Stem Cells from Human Extraocular Muscle Tissue
Sharma A, et al.
Bio-protocol, 9(4) (2016)
Paul A. Banaszkiewicz
Classic Papers in Orthopaedics (2014)
Perturbations in the HDL metabolic pathway predispose to the development of osteoarthritis in mice following long-term exposure to western-type diet
I.-E.Triantaphyllidou
Osteoarthritis and Cartilage / OARS, Osteoarthritis Research Society (2013)
Novel markers of osteogenic and adipogenic differentiation of human bone marrow stromal cells identified using a quantitative proteomics approach
CeciliaGraneli
Stem Cell Research (2014)
Measurement of cytochrome P450 and NADPH-cytochrome P450 reductase
Guengerich FP, et al.
Nature Protocols, 4(9), 1245-1245 (2009)
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