SRP3016
BMP-4 human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
BMP-2B, DVR4
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Product Name
BMP-4 human, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
5-10 ng/mL ED50
mol wt
23.8 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... BMP4(652)
Application
BMP-4 human has been used for in vitro chondrogenesis in goat adipose tissue-derived mesenchymal stem cells.
Biochem/physiol Actions
Members of TGF-β (transforming growth factor β) superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life. Like BMP-7, BMP-4 (bone morphogenetic protein 4) is involved in the development and maintenance of bone and cartilage. Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. BMP-4 controls the expression of miRNAs in breast cancer cell lines, thereby regulating the proliferation and migration phenotypes. It is involved in carcinogenesis and exhibits its effect through the SMAD-dependent signaling. In mice, homozygous mutations in BMP4 cause early embryonic lethality.
General description
Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β (transforming growth factor β) superfamily of structurally related signaling proteins. Escherichia coli derived BMP-4 is a fully active homodimeric protein consisting of two 106 amino acid subunits which correspond to amino acids 303-408 of the full length BMP-4 precursor.
Other Notes
KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in 5 mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8° C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20° C to -80° C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Bone morphogenetic protein 4 regulates microRNA expression in breast cancer cell lines in diverse fashion.
Alarmo EL
Genes Chromosomes Cancer, 55, 227-236 (2016)
Transgenic mice overexpressing BMP4 develop a fibrodysplasia ossificans progressiva (FOP)-like phenotype.
Kan L
The American Journal of Pathology, 165, 1107-1115 (2004)
Generation of handmade cloned embryos from adipose tissue derived mesenchymal stem cells in goat.
Malik HN
Small Ruminant Research, 121, 340-350 (2014)
Applications of small molecule BMP inhibitors in physiology and disease.
Hong CC and Yu PB
Cytokine & Growth Factor Reviews, 20, 409-418 (2009)
Overexpression of the BMP4/SMAD signaling pathway in skull base chordomas is associated with poor prognosis.
Feng Y
International Journal of Clinical and Experimental Pathology, 8, 8268-8275 (2015)
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