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Merck

T2507

Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

Synonym(s):

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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About This Item

CAS Number:
9023-78-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-774-2
MDL number:
MFCD00082080
EC Number:
5.3.1.1 (BRENDA, IUBMB)
Specific activity:
~10,000 units/mg protein

Key Documents

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type

Type I

form

ammonium sulfate suspension

specific activity

~10,000 units/mg protein

mol wt

53 kDa by equilibrium centrifugation

foreign activity

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%

storage temp.

2-8°C

Quality Level

200

Related Categories

Application

Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.

Physical form

Crystalline suspension in 2.7 M (NH4)2SO4, 0.5 mM EDTA, pH 6.5

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.
Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
036H8025
060H8110

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Xiao Ma et al.
Electrophoresis, 33(9-10), 1349-1355 (2012-06-01)
Prefractionation of proteins enhances the resolution of proteome analysis of whole cells. Free-flow electrophoresis (FFE) provides a useful step in various prefractionation protocols, since matrix-free isoelectric focusing (FF-IEF) performed in this machine enables the enrichment of large, easily absorbable, sensitive
Annett Linge et al.
Investigative ophthalmology & visual science, 53(8), 4634-4643 (2012-05-10)
To compare the proteomic profiles of two categories of primary uveal melanoma tissue samples; those from patients who have subsequently developed metastatic disease and those who have not. Two-dimensional difference gel electrophoresis (2D DIGE) was performed on 25 uveal melanoma
Xiaochen He et al.
Journal of cellular physiology, 237(8), 3317-3327 (2022-05-28)
Hypertension is an important risk factor in the pathogenesis of diastolic dysfunction. Growing evidence indicates that glucose metabolism plays an essential role in diastolic dysfunction. TP53-induced glycolysis and apoptosis regulator (TIGAR) has been shown to regulate glucose metabolism and heart
Rebecca Blomberg et al.
Nature, 503(7476), 418-421 (2013-10-18)
Linus Pauling established the conceptual framework for understanding and mimicking enzymes more than six decades ago. The notion that enzymes selectively stabilize the rate-limiting transition state of the catalysed reaction relative to the bound ground state reduces the problem of
Rongchang Yang et al.
Experimental parasitology, 137, 46-52 (2013-12-21)
A novel quantitative PCR (qPCR) for Giardia at the glutamate dehydrogenase (gdh) locus was developed and validated. The qPCR was used to screen a total of 3412 lamb faecal samples collected from approximately 1189 lambs at three sampling periods (weaning
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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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