T3413
Monoclonal Anti-Tenascin antibody produced in rat
clone MTn-12, ascites fluid
Synonym(s):
Anti-Tenascin-N
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About This Item
Product Name
Monoclonal Anti-Tenascin antibody produced in rat, clone MTn-12, ascites fluid
biological source
rat
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
MTn-12, monoclonal
contains
15 mM sodium azide
species reactivity
mouse
technique(s)
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:200 using unfixed, frozen tissue sections of mouse intestine
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Tnn(329278)
Biochem/physiol Actions
Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. It was proposed that actively growing, migrating and differentiating epithelial sheets can produce factors that can stimulate tenascin expression in the nearby mesenchyme. Human and chicken tenascin contain an RGD sequence motif which may function in cell adhesion and may be recognized by integrin receptor.
The antibody localizes mouse tenascin in the supernatant of cultured mouse fibroblasts and tissue extracts. No cross-reactivity with tenascin of other species has been observed. In immunohistological testing of frozen tissue sections of mouse intestine, the antibody labels the core of the villi, but not the epithelial cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Mouse Tenascin (rat IgG1 isotype) is derived from the MTn-12 hybridoma1 produced by the fusion of rat myeloma cells and splenocytes from a Lou rat immunized with partially purified mouse tenascin. Human tenascin has three subunits of 190, 200 and 220 kDa. Tenascin has been independently discovered in a variety of species and tissue types, often in the basement membrane or intercellular spaces. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer, depending on species, the molecular weights of the subunits range from 190 to 320 kDa.
Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM).
The tenascin molecule is a disulfide-linked hexamer; depending on species, the molecular weights of the subunits range from 190 to 320 kDa. In the mouse, two major subunits of tenascin with an apparent molecular weight of 210 and 260 kDa have been described. The shorter polypeptide predominates during earlier developmental stages and the larger polypeptide appears later in the embryonic gut and especially in the adult intestine. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. It was proposed that actively growing, migrating and differentiating epithelial sheets can produce factors that can stimulate tenascin expression in the nearby mesenchyme. Human and chicken tenascin contain an RGD sequence which may function in cell adhesion and it seems likely that tenascin mediates cell attachment through an RGD dependent integrin receptor.
The tenascin molecule is a disulfide-linked hexamer; depending on species, the molecular weights of the subunits range from 190 to 320 kDa. In the mouse, two major subunits of tenascin with an apparent molecular weight of 210 and 260 kDa have been described. The shorter polypeptide predominates during earlier developmental stages and the larger polypeptide appears later in the embryonic gut and especially in the adult intestine. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. It was proposed that actively growing, migrating and differentiating epithelial sheets can produce factors that can stimulate tenascin expression in the nearby mesenchyme. Human and chicken tenascin contain an RGD sequence which may function in cell adhesion and it seems likely that tenascin mediates cell attachment through an RGD dependent integrin receptor.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-Mouse Tenascin antibody may be used for the localization of tenascin and to study of the role of tenascin in epithelial-mesenchymal interactions using various immunochemical assays including ELISA, immunoblot, dot blot and immunohistology.
Monoclonal Anti-Tenascin antibody produced in rat has been used in:
- Enzyme linked immunosorbent assay (ELISA)
- Dot blot.
- Immunoblotting
- Fluorescence microscopy and immunostaining
- Immunofluorescence
- Immunohistochemistry
Immunogen
partially purified mouse tenascin.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Transgenic overexpression of the alpha7 integrin reduces muscle pathology and improves viability in the dyW mouse model of merosin-deficient congenital muscular dystrophy type 1A
Doe JA, et al.
Journal of Cell Science, 124(13), 2287-2297 (2011)
Developmental changes in patterns of expression of tenascin-C variants in the human cornea
Maseruka H, et al.
Investigative Ophthalmology & Visual Science, 41(13), 4101-4107 (2000)
Clinical impact and functional aspects of tenascin-C expression during glioma progression
Herold-Mende C, et al.
International Journal of Cancer. Journal International Du Cancer, 98(3), 362-369 (2002)
The Expression and Possible Functions of Tenascin-W During Development and Disease
Tucker RP and Degen M
Frontiers in Cell and Developmental Biology, 7, 53-53 (2019)
Hox11 genes are required for regional patterning and integration of muscle, tendon and bone
Swinehart IT, et al.
Development, 140(22), 4574-4582 (2013)
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