3,3′,5,5′-Tetramethylbenzidine

Name: 3,3&#8242;,5,5&#8242;-Tetramethylbenzidine
Brand: SIGMA

TMB substrate, chromogenic, tablet

Synonym(s):

BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate

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About This Item

Linear Formula:

[-C₆H₂(CH₃)₂-4-NH₂]₂

CAS Number:

54827-17-7

Molecular Weight:

240.34

NACRES:

NA.83

PubChem Substance ID:

24900293

UNSPSC Code:

12352204

MDL number:

MFCD00007748

Beilstein/REAXYS Number:

2808541

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Properties

Product Name

3,3′,5,5′-Tetramethylbenzidine, tablet, 1 mg substrate per tablet

Quality Level

200

form

tablet

mp

168-171 °C (lit.)

storage temp.

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

3,3′,5,5′-Tetramethylbenzidine has been used as a substrate in competitive enzyme-linked immunosorbent assay (ELISA) and indirect ELISA.